| RRC ID |
84966
|
| 著者 |
Ikeda Y, Miyazaki R, Tsukazaki T, Akiyama Y, Mori H.
|
| タイトル |
Translation arrest cancellation of VemP, a secretion monitor in Vibrio, is regulated by multiple cis and trans factors, including SecY.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
VemP is a secretory protein in the Vibrio species that monitors cellular protein-transport activity through its translation arrest, allowing expression of the downstream secD2-secF2 genes in the same operon, which encode components of the protein translocation machinery. When cellular protein-transport function is fully active, secD2/F2 expression remains repressed as VemP translation arrest is canceled immediately. The VemP arrest cancellation occurs on the SecY/E/G translocon in a late stage in the translocation process and requires both trans factors, SecD/F and PpiD/YfgM, and a cis element, Arg-85 in VemP; however, the detailed molecular mechanism remains elusive. This study aimed to elucidate how VemP passing through SecY specifically monitors SecD/F function. Genetic and biochemical studies showed that SecY is involved in the VemP arrest cancellation and that the arrested VemP is stably associated with a specific site in the protein-conducting pore of SecY. VemP-Bla reporter analyses revealed that a short hydrophobic segment adjacent to Arg-85 plays a critical role in the regulated arrest cancellation with its hydrophobicity correlating with the stability of the VemP arrest. We identified Gln-65 and Pro-67 in VemP as novel elements important for the regulation. We propose a model for the regulation of the VemP arrest cancellation by multiple cis elements and trans factors with different roles.
|
| 巻・号 |
300(10)
|
| ページ |
107735
|
| 公開日 |
2024-10-1
|
| DOI |
10.1016/j.jbc.2024.107735
|
| PII |
S0021-9258(24)02236-1
|
| PMID |
39233231
|
| PMC |
PMC11470409
|
| MeSH |
Bacterial Proteins* / genetics
Bacterial Proteins* / metabolism
Gene Expression Regulation, Bacterial*
Protein Biosynthesis
Protein Transport
Vibrio / genetics
Vibrio / metabolism
Vibrio cholerae / genetics
Vibrio cholerae / metabolism
|
| IF |
4.238
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9012
MC4100 |
