| RRC ID |
85000
|
| Author |
Stig Andersen, Johan Quilbé, Troels Mouritzen, Turgut Akyol, Yusdar Mustamin, Masaru Bamba, Mikkel Schierup, Shusei Sato
|
| Title |
Microbiome interactions increase plant haplotype richness
|
| Abstract |
The genetic makeup of natural plant populations often allows them to persist for many years without succumbing to disease. Transferring such properties to crops could increase resilience and reduce reliance on chemical pesticides. However, the population genomics of plant-microbiome interactions remain poorly understood. Here, we use Lotus japonicus, which has persisted as a natural population in Japan for ~20’000 years, to identify genomic selection signatures caused by soil microbes. We found strong genetic associations with root microbiome structure within a gene we name ROOT MICROBIOME ESTABLISHMENT 1 (ROOMIE1) located in a region with high haplotype richness. This led us to quantify haplotype richness genome-wide using a new metric, HAPk. We found haplotype-rich regions strongly enriched for microbiome genetic associations, suggesting that soil microbes were imposing negative frequency-dependent selection. We validated this hypothesis by showing increased migration rates of microbiome associated alleles and by determining that ROOMIE1 shows standing variation in local populations and impacts rhizosphere colonization in native Japanese soil. Our results indicate that interactions with soil microbes represent a major selective force in plant genome evolution and suggest that haplotype-rich regions constitute a genetic resource for improvement of crop resilience.
|
| DOI |
10.21203/rs.3.rs-5130034/v1
|
| Resource |
| Lotus / Glycine |
LegumeBase |
