論文 - 詳細
| RRC ID | 85011 |
|---|---|
| 著者 | Kurawaki S, Nakashima A, Ishiuchi N, Kanai R, Maeda S, Sasaki K, Masaki T. |
| タイトル | Mesenchymal stem cells pretreated with interferon-gamma attenuate renal fibrosis by enhancing regulatory T cell induction. |
| ジャーナル | Sci Rep |
| Abstract |
Mesenchymal stem cells (MSCs) exert their anti-inflammatory and anti-fibrotic effects by secreting various humoral factors. Interferon-gamma (IFN-γ) can enhance these effects of MSCs, and enhancement of regulatory T (Treg) cell induction is thought to be an underlying mechanism. However, the extent to which Treg cell induction by MSCs pretreated with IFN-γ (IFN-γ MSCs) ameliorates renal fibrosis remains unknown. In this study, we investigated the effects of Treg cell induction by IFN-γ MSCs on renal inflammation and fibrosis using an siRNA knockdown system. Administration of IFN-γ MSCs induced Treg cells and inhibited infiltration of inflammatory cells in ischemia reperfusion injury (IRI) rats more drastically than control MSCs without IFN-γ pretreatment. In addition, administration of IFN-γ MSCs more significantly attenuated renal fibrosis compared with control MSCs. Indoleamine 2,3-dioxygenase (IDO) expression levels in conditioned medium from MSCs were enhanced by IFN-γ pretreatment. Moreover, IDO1 knockdown in IFN-γ MSCs reduced their anti-inflammatory and anti-fibrotic effects in IRI rats by reducing Treg cell induction. Our findings suggest that the increase of Treg cells induced by enhanced secretion of IDO by IFN-γ MSCs played a pivotal role in their anti-fibrotic effects. Administration of IFN-γ MSCs may potentially be a useful therapy to prevent renal fibrosis progression. |
| 巻・号 | 14(1) |
| ページ | 10251 |
| 公開日 | 2024-5-4 |
| DOI | 10.1038/s41598-024-60928-4 |
| PII | 10.1038/s41598-024-60928-4 |
| PMID | 38704512 |
| PMC | PMC11069572 |
| MeSH | Animals Fibrosis* Indoleamine-Pyrrole 2,3,-Dioxygenase* / metabolism Interferon-gamma* / metabolism Kidney / drug effects Kidney / pathology Kidney Diseases / pathology Kidney Diseases / therapy Male Mesenchymal Stem Cell Transplantation* / methods Mesenchymal Stem Cells* / metabolism Rats Rats, Sprague-Dawley Reperfusion Injury / immunology T-Lymphocytes, Regulatory* / immunology |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Human MSCs |