論文 - 詳細
| RRC ID | 85086 |
|---|---|
| 著者 | Tamura M, Yamashita W, Hibi T, Inui S, Tanaka K, Ozako M, Shimasaki T, Ohtsuka H, Shibuya M, Yamamoto Y, Yokoshima S, Aiba H. |
| タイトル | Characterization of P-type H+-ATPase Pma1 inhibitors that extend chronological lifespan in fission yeast. |
| ジャーナル | Mol Genet Genomics |
| Abstract |
Inhibition of the activity of Pma1, a widely conserved P-type proton exporting ATPase, has been shown to extend the chronological lifespan (CLS) in fission yeast Schizosaccharomyces pombe. To develop a specific inhibitor for Pma1 of S. pombe, we focused on Si01, a candidate inhibitor of Saccharomyces cerevisiae Pma1. First, we have established a method for synthesis of Si01 and then investigated its Pma1 inhibitory activity and lifespan extension effect in fission yeast. Second, we also synthesized derivatives of Si01 and determined the minimum structure required for inhibition of S. pombe Pma1. Here we showed that the inhibitory activity of Pma1 correlates with the effect of lifespan extension. Si01 reduced the activity of purified Pma1 protein and extended the CLS of not only fission yeast but also budding yeast. These results provide a molecular basis for understanding the mechanism of Pma1 inhibition and the potential for developing molecules that regulate lifespan. |
| 巻・号 | 300(1) |
| ページ | 58 |
| 公開日 | 2025-6-8 |
| DOI | 10.1007/s00438-025-02264-4 |
| PII | 10.1007/s00438-025-02264-4 |
| PMID | 40484900 |
| MeSH | Enzyme Inhibitors* / chemical synthesis Enzyme Inhibitors* / chemistry Enzyme Inhibitors* / pharmacology Proton-Translocating ATPases* / antagonists & inhibitors Proton-Translocating ATPases* / genetics Proton-Translocating ATPases* / metabolism Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins* / antagonists & inhibitors Saccharomyces cerevisiae Proteins* / genetics Saccharomyces cerevisiae Proteins* / metabolism Schizosaccharomyces* / drug effects Schizosaccharomyces* / enzymology Schizosaccharomyces* / genetics Schizosaccharomyces pombe Proteins* / antagonists & inhibitors Schizosaccharomyces pombe Proteins* / metabolism |
| IF | 2.797 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
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