Reference - Detail
| RRC ID | 85168 |
|---|---|
| Author | Berraquero M, Tallada VA, Jimenez J. |
| Title | Ltc1 localization by EMC regulates cell membrane fluidity to facilitate membrane protein biogenesis. |
| Journal | iScience |
| Abstract |
The EMC complex, a highly conserved transmembrane chaperone in the endoplasmic reticulum (ER), has been associated in humans with sterol homeostasis and a myriad of different cellular activities, rendering the mechanism of EMC functionality enigmatic. Using fission yeast, we demonstrate that the EMC complex facilitates the biogenesis of the sterol transfer protein Lam6/Ltc1 at ER-plasma membrane and ER-mitochondria contact sites. Cells that lose EMC function sequester unfolded Lam6/Ltc1 and other proteins at the mitochondrial matrix, leading to surplus ergosterol, cold-sensitive growth, and mitochondrial dysfunctions. Remarkably, inhibition of ergosterol biosynthesis, but also fluidization of cell membranes to counteract their rigidizing effects, reduce the ER-unfolded protein response and rescue growth and mitochondrial defects in EMC-deficient cells. These results suggest that EMC-assisted biogenesis of Lam6/Ltc1 may provide, through ergosterol homeostasis, optimal membrane fluidity to facilitate biogenesis of other ER-membrane proteins. |
| Volume | 28(3) |
| Pages | 112096 |
| Published | 2025-3-21 |
| DOI | 10.1016/j.isci.2025.112096 |
| PII | S2589-0042(25)00356-6 |
| PMID | 40124504 |
| PMC | PMC11928854 |
| IF | 4.447 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Blogs |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | FY33691 FY17341 |