論文 - 詳細
| RRC ID | 85171 |
|---|---|
| 著者 | Berraquero M, Tallada VA, Jimenez J. |
| タイトル | A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts. |
| ジャーナル | Yeast |
| Abstract |
In eukaryotes, oxygen consumption is mainly driven by the respiratory activity of mitochondria, which generates most of the cellular energy that sustains life. This parameter provides direct information about mitochondrial activity of all aerobic biological systems. Using the Seahorse analyzer instrument, we show here that deletion of the oca3/emc2 gene (oca3Δ) encoding the Emc2 subunit of the ER membrane complex (EMC), a conserved chaperone/insertase that aids membrane protein biogenesis in the ER, severely affects oxygen consumption rates and quiescence survival in Schizosaccharomyces pombe yeast cells. Remarkably, the respiratory defect of the oca3Δ mutation (EMC dysfunction) is rescued synergistically by disruption of ergosterol biosynthesis (erg5Δ) and the action of the membrane fluidizing agent tween 20, suggesting a direct role of membrane fluidity and sterol composition in mitochondrial respiration in the fission yeast. |
| 巻・号 | 42(4) |
| ページ | 96-103 |
| 公開日 | 2025-4-1 |
| DOI | 10.1002/yea.3998 |
| PMID | 40085054 |
| MeSH | Endoplasmic Reticulum / metabolism Ergosterol / biosynthesis Gene Deletion Membrane Proteins* / genetics Membrane Proteins* / metabolism Mitochondria* / metabolism Oxygen Consumption* Schizosaccharomyces* / genetics Schizosaccharomyces* / metabolism Schizosaccharomyces* / physiology Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism |
| IF | 3.143 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY33691 FY17341 |