| RRC ID |
85260
|
| Author |
Kurosu H, Yamada N, Nakamura R, Ito H, Ohnishi K, Inoko A, Riku M, Muramatsu T, Sassa N, Kasai K.
|
| Title |
AEBP1-GLI1 pathway attenuates the FACT complex dependency of bladder cancer cell survival.
|
| Journal |
Biochem Biophys Rep
|
| Abstract |
The facilitates chromatin transcription (FACT) complex is composed of SSRP1 and SUPT16H subunits and participates in nucleosomal reorganization; hence, FACT inhibitors are considered promising therapeutics for malignant tumors. Here, we show that adipocyte enhancer binding protein 1 (AEBP1) attenuates the dependency of bladder cancer cell survival on the FACT complex via the expression of GLI1, a pivotal transcription factor in Hedgehog signaling. In AEBP1-high expressing bladder cancer cell lines, AEBP1 knockdown inhibited cellular proliferation and induced the marker expression of apoptosis and DNA damage/replication stress. RNA-sequencing revealed that AEBP1 knockdown suppressed the expression of SSRP1 and SUPT16H; however, the knockdown of both subunits was less effective than AEBP1 knockdown in inducing apoptosis or DNA damage markers in AEBP1-high expressing cells. AEBP1 knockdown reduced the protein levels of GLI1, and treatment with the GLI-specific inhibitor GANT61 induced markers that were not suppressed by the forced expression of AEBP1. These findings suggest that AEBP1-mediated GLI1 expression reduces the FACT complex dependency of bladder cancer cell survival.
|
| Volume |
43
|
| Pages |
102101
|
| Published |
2025-9-1
|
| DOI |
10.1016/j.bbrep.2025.102101
|
| PII |
S2405-5808(25)00188-8
|
| PMID |
40612002
|
| PMC |
PMC12221834
|
| Resource |
| DNA material |
CSII-CMV-MCS-IRES2-Bsd (RDB04385)
pCMV-VSV-G-RSV-Rev (RDB04393)
pCAG-HIVgp (RDB04394) |
| Human and Animal Cells |
JMSU1(RCB2227) |
