Reference - Detail
| RRC ID | 85343 |
|---|---|
| Author | Goto T, Nakano M, Danno S, Ueda C, Sakaue-Sawano A, Miyawaki A, Wrabel A, Nakahara I, Nishikata T, Mizoguchi A, Tamura Y, Mizuno K, Kataoka Y, Funabiki K. |
| Title | Microendoscopy for periodic intravital end-to-end tumor imaging of cancer cells. |
| Journal | Cell Rep Methods |
| Abstract |
The spatiotemporal heterogeneity in intratumor proliferative behavior of cancer cells deeply affects tumor environment characteristics and the efficacy of anticancer treatments. Thus, intravital imaging with unlimited imaging depth and cellular-level resolution is greatly desired. We developed an optical-fiber-bundle-based microendoscope with a genetically encoded fluorescent ubiquitination-based cell-cycle indicator (Fucci) system to achieve the intravital, periodic, and multicolor end-to-end imaging of the proliferative activity of cancer cells at a cellular-level resolution. This technique enabled the periodic visualization of spatiotemporal cellular responses, including cell-cycle arrest and resumption, and nuclear enlargement following the administration of anticancer drugs in living mice. It was suggested that proliferating cell ratio and nuclear enlargement in cancer cells at the surface region of tumor characterized by abundant vascular invasion contribute to aggressive tumor regrowth after chemotherapy. The application of this technique can accelerate innovation in cancer biology and therapeutics. |
| Volume | 5(6) |
| Pages | 101056 |
| Published | 2025-6-16 |
| DOI | 10.1016/j.crmeth.2025.101056 |
| PII | S2667-2375(25)00092-X |
| PMID | 40472849 |
| PMC | PMC12272250 |
| MeSH | Animals Cell Line, Tumor Cell Proliferation / drug effects Endoscopy* / methods Humans Intravital Microscopy* / methods Mice Mice, Nude Neoplasms* / diagnostic imaging Neoplasms* / pathology |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | C6(RCB2854) |