論文 - 詳細
| RRC ID | 85372 |
|---|---|
| 著者 | Yoshida T, Monma C, Ninomiya Y, Takiguchi S, Fujita S, Uchida Y, Sakoda N, Karginov VA, Kishikawa JI, Yamada T, Kawano R, Tsuge H. |
| タイトル | Serine clamp of Clostridium perfringens binary toxin BECb (CPILEb)-pore confers cytotoxicity and enterotoxicity. |
| ジャーナル | Commun Biol |
| Abstract |
BEC (CPILE) is a virulence factor of the pathogen, Clostridium perfringens, which has caused foodborne outbreaks in Japan. BEC is a binary toxin that comprises the enzymatic A-component (BECa) and the B-component (BECb); the latter forms a membrane pore to translocate the A-component into target cells. Although BEC differs from other binary toxins in that the B-component alone shows enterotoxic activity, the reason for this remains unclear. We focus on the narrowest region of BECb-pore formed by not phenylalanine residues conserved in other binary toxins including iota toxin B-component (Ib) but serine residues. Comparisons between BECb and BECb (S405F) where the serine residue forming the narrowest region is substituted to the phenylalanine residue reveal that the serine residue is responsible for both cytotoxicity and enterotoxic activity. Though attempts to prepare the BECb-pore were unsuccessful, we reveal the cryo-EM structure of Ib (F454S) where the phenylalanine residue forming the narrowest region is substituted to the serine residue as a surrogate of BECb. Furthermore, Ib (F454S) increases current conductance to nine times that of Ib due to the larger pore diameter and the hydrophilic nature. These results suggest that BECb functions as a pore-forming toxin and as a translocation channel for BECa. |
| 巻・号 | 8(1) |
| ページ | 1102 |
| 公開日 | 2025-7-25 |
| DOI | 10.1038/s42003-025-08519-5 |
| PII | 10.1038/s42003-025-08519-5 |
| PMID | 40715636 |
| PMC | PMC12297211 |
| MeSH | Animals Bacterial Proteins* / chemistry Bacterial Proteins* / genetics Bacterial Proteins* / metabolism Bacterial Toxins* / chemistry Bacterial Toxins* / genetics Bacterial Toxins* / metabolism Bacterial Toxins* / toxicity Clostridium perfringens* / genetics Clostridium perfringens* / metabolism Clostridium perfringens* / pathogenicity Cryoelectron Microscopy Humans Serine* / chemistry Serine* / metabolism |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 28 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 2.3 |
| リソース情報 | |
| ヒト・動物細胞 | A431(RCB0202) L929(RCB1422) MDCK(RCB0995) |