Reference - Detail
| RRC ID | 85375 |
|---|---|
| Author | Ukita Y, Suzuki R, Miyoshi K, Saito K, Okumura M, Chihara T. |
| Title | Generation of Odorant Receptor-QF2 Knock-In Drivers for Improved Analysis of Olfactory Circuits in Drosophila. |
| Journal | Genes Cells |
| Abstract |
Drosophila melanogaster has provided numerous insights into the olfactory system, primarily relying on a series of transgenic Gal4 drivers. The combined use of Gal4/UAS and a second binary expression system, such as the QF/QUAS system, provides the opportunity to manipulate the two distinct cell populations, thereby accelerating the elucidation of the olfactory neural mechanisms. However, resources apart from the Gal4/UAS system have been poorly developed. In this study, we generated a series of odorant receptor (Or)-QF2 knock-in driver (Or-QF2KI) lines for 23 Ors using the CRISPR/Cas9 knock-in method. In these lines, the QF2 protein is cotranslated with each Or product. The expression pattern of the Or-QF2KI drivers mostly corresponded to that of the Or-Gal4 drivers. In addition, the Or42a-QF2KI driver identified the additional expression pattern of Or42a, which is consistent with the data of single-nucleus RNA sequencing and is attributed to the Or-QF2KI drivers' ability to reflect the endogenous expression of the Or genes. Thus, these Or-QF2KI drivers can be used as valuable genetic tools for olfactory research in Drosophila. |
| Volume | 30(4) |
| Pages | e70028 |
| Published | 2025-7-1 |
| DOI | 10.1111/gtc.70028 |
| PMID | 40439284 |
| PMC | PMC12121335 |
| MeSH | Animals Animals, Genetically Modified CRISPR-Cas Systems Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / genetics Drosophila melanogaster* / metabolism Gene Knock-In Techniques / methods Olfactory Pathways* / metabolism Olfactory Receptor Neurons / metabolism Receptors, Odorant* / genetics Receptors, Odorant* / metabolism |
| IF | 1.655 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | CAS-0011 CAS-0012 DGRC#119657 DGRC#119658 DGRC#119659 DGRC#119660 DGRC#119661 DGRC#119662 DGRC#119663 DGRC#119664 DGRC#119665 DGRC#119666 DGRC#119667 DGRC#119668 DGRC#119669 DGRC#119670 DGRC#119671 DGRC#119672 DGRC#119673 DGRC#119674 DGRC#119675 DGRC#119676 DGRC#119677 DGRC#119678 DGRC#119679 |