論文 - 詳細
| RRC ID | 85388 |
|---|---|
| 著者 | Kojima T, Hayashi T, Kageyama Y, Nakamura T, Akiyama T. |
| タイトル | Exosome-like nanovesicles from Peucedanum Japonicum directly regulate inflammatory cytokines via small RNAs. |
| ジャーナル | Sci Rep |
| Abstract |
Inflammation plays a key role in the pathogenesis of various diseases, including inflammatory bowel diseases such as ulcerative colitis and Crohn's disease. Similar to animals, plant cells produce exosome-like nanovesicles (ELNs) that play a role in transmitting biological signals from specific types of cells or tissues to other cells or tissues. Here we show that ELNs derived from Peucedanum japonicum (PjELNs) exhibit potent anti-inflammatory effects on macrophages and are effective in a mouse colitis model induced by dextran sodium sulfate (DSS). RNA sequence analysis reveals that PjELNs suppress multiple inflammatory cytokine-mediated signaling pathways in LPS-stimulated macrophage cell lines. We further show that PjENLs promote the differentiation of goblet cells, thereby enhancing mucin production and providing a protective effect on mucosal damage in a DSS-induced murine model of colitis. We also demonstrate that PjELNs inhibit the DSS-induced reduction in the number of transit amplifying cells. PjELNs exhibit anti-inflammatory properties, partly due to the presence of miRNA-like small RNAs that directly regulate the expression of the inflammatory cytokine IL6. Importantly, these small RNAs exhibit cross-species effects in both humans and mice. These findings reveal the mechanism by which plant-derived ELNs modulate inflammatory responses, suggesting their potential as a preventive and therapeutic strategy for inflammatory diseases of the colon. |
| 巻・号 | 15(1) |
| ページ | 27424 |
| 公開日 | 2025-7-28 |
| DOI | 10.1038/s41598-025-12175-4 |
| PII | 10.1038/s41598-025-12175-4 |
| PMID | 40721445 |
| PMC | PMC12304193 |
| MeSH | Animals Anti-Inflammatory Agents* / pharmacology Colitis / chemically induced Colitis / drug therapy Colitis / metabolism Colitis / pathology Cytokines* / metabolism Dextran Sulfate Disease Models, Animal Exosomes* / metabolism Humans Inflammation Macrophages / drug effects Macrophages / metabolism Mice Mice, Inbred C57BL MicroRNAs* / genetics |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |