| RRC ID |
85388
|
| 著者 |
Kojima T, Hayashi T, Kageyama Y, Nakamura T, Akiyama T.
|
| タイトル |
Exosome-like nanovesicles from Peucedanum Japonicum directly regulate inflammatory cytokines via small RNAs.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Inflammation plays a key role in the pathogenesis of various diseases, including inflammatory bowel diseases such as ulcerative colitis and Crohn's disease. Similar to animals, plant cells produce exosome-like nanovesicles (ELNs) that play a role in transmitting biological signals from specific types of cells or tissues to other cells or tissues. Here we show that ELNs derived from Peucedanum japonicum (PjELNs) exhibit potent anti-inflammatory effects on macrophages and are effective in a mouse colitis model induced by dextran sodium sulfate (DSS). RNA sequence analysis reveals that PjELNs suppress multiple inflammatory cytokine-mediated signaling pathways in LPS-stimulated macrophage cell lines. We further show that PjENLs promote the differentiation of goblet cells, thereby enhancing mucin production and providing a protective effect on mucosal damage in a DSS-induced murine model of colitis. We also demonstrate that PjELNs inhibit the DSS-induced reduction in the number of transit amplifying cells. PjELNs exhibit anti-inflammatory properties, partly due to the presence of miRNA-like small RNAs that directly regulate the expression of the inflammatory cytokine IL6. Importantly, these small RNAs exhibit cross-species effects in both humans and mice. These findings reveal the mechanism by which plant-derived ELNs modulate inflammatory responses, suggesting their potential as a preventive and therapeutic strategy for inflammatory diseases of the colon.
|
| 巻・号 |
15(1)
|
| ページ |
27424
|
| 公開日 |
2025-7-28
|
| DOI |
10.1038/s41598-025-12175-4
|
| PII |
10.1038/s41598-025-12175-4
|
| PMID |
40721445
|
| PMC |
PMC12304193
|
| MeSH |
Animals
Anti-Inflammatory Agents* / pharmacology
Colitis / chemically induced
Colitis / drug therapy
Colitis / metabolism
Colitis / pathology
Cytokines* / metabolism
Dextran Sulfate
Disease Models, Animal
Exosomes* / metabolism
Humans
Inflammation
Macrophages / drug effects
Macrophages / metabolism
Mice
Mice, Inbred C57BL
MicroRNAs* / genetics
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
293T(RCB2202) |
