論文 - 詳細
| RRC ID | 85434 |
|---|---|
| 著者 | Kamuro R, Yoshihara M, Hara S, Nemoto T, Howard L, Honda A, Yamamoto A, Takigawa T, Sakimoto S, Baba K, Quantock AJ, Nishida K. |
| タイトル | PAX6-dependent differentiation landscape of ocular surface epithelium via single-cell RNA sequencing in hiPSC-derived ocular developmental organoid. |
| ジャーナル | Commun Biol |
| Abstract |
During eye development, surface ectoderm cells that express PAX6 differentiate into corneal, limbal and conjunctival epithelia. However, several aspects of this differentiation process -- such as the developmental origin of the limbal epithelium and the mechanisms that underlie PAX6-mediated lineage specification -- are not properly understood. To explore these issues, we used single-cell RNA sequencing to study ocular surface epithelial cells derived from human induced pluripotent stem cells. Our analysis reveals that the corneal and conjunctival epithelial cell lineages originate from the surface ectoderm, and that the conjunctival lineage contributes to limbal epithelial cell populations. We also show that primordial conjunctival epithelial cells express limbal epithelial markers before lineage bifurcation. Finally, the activity and expression of PAX6 are highest in corneal epithelial cells, followed by those of the limbal epithelium and conjunctival epithelium, suggesting that lineage-specific differentiation is regulated by levels of PAX6 activity. These findings provide deeper understanding of the early-stage human ocular surface development. |
| 巻・号 | 8(1) |
| ページ | 1220 |
| 公開日 | 2025-8-14 |
| DOI | 10.1038/s42003-025-08643-2 |
| PII | 10.1038/s42003-025-08643-2 |
| PMID | 40813888 |
| PMC | PMC12354803 |
| MeSH | Cell Differentiation* Cell Lineage Conjunctiva / cytology Conjunctiva / metabolism Epithelial Cells / cytology Epithelial Cells / metabolism Epithelium, Corneal* / cytology Epithelium, Corneal* / metabolism Gene Expression Regulation, Developmental Humans Induced Pluripotent Stem Cells* / cytology Induced Pluripotent Stem Cells* / metabolism Organoids* / cytology Organoids* / metabolism PAX6 Transcription Factor* / genetics PAX6 Transcription Factor* / metabolism Sequence Analysis, RNA Single-Cell Analysis |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |
| 遺伝子材料 | TP63-tdTomato-Neo (RDB21101) |