論文 - 詳細
| RRC ID | 85462 |
|---|---|
| 著者 | Sakoshita K, Aratani S, Kameda N, Takebe R, Tominaga T, Ishida M, Hori M. |
| タイトル | Anoctamin-like protein 1 regulates repolarization in Paramecium behavioral responses. |
| ジャーナル | J Eukaryot Microbiol |
| Abstract |
Paramecium exhibits responsive behavior to environmental changes, moving either closer to or further away from stimuli. Electrophysiological experiments have revealed that these behavioral responses are controlled by membrane potentials. Anoctamin, a Ca2+-activated Cl- channel, is involved in the regulation of membrane potential in mammals. However, it remains uncertain whether Cl- channels like anoctamin regulate Paramecium behavior. Herein, replacement of external Cl- ions with acetate ion and application of Cl- channel blocker niflumic acid (NFA, 0.1 μM) increased spontaneous avoiding reactions (sARs). Hence, we hypothesized that anoctamin is involved in the stabilization of membrane potential fluctuation. Paramecium cells in which the anoctamin-like protein 1 gene was knocked down displayed frequent sARs in the culture medium without external stimulation. Treatment of anoctamin-like protein 1-knockdown cells with the Ca2+ chelator BAPTA or Ca-channel blocker nicardipine reversed the increase in sARs. Electrophysiological experiments revealed extension of membrane depolarization when positive currents were applied to anoctamin-like protein 1-knockdown cells. We concluded that anoctamin-like protein 1 works as a Cl-channel and stabilizes the membrane potential oscillation, reducing sARs. |
| 巻・号 | 71(4) |
| ページ | e13030 |
| 公開日 | 2024-1-1 |
| DOI | 10.1111/jeu.13030 |
| PMID | 38757880 |
| MeSH | Anoctamins* / genetics Anoctamins* / metabolism Calcium / metabolism Chloride Channels / antagonists & inhibitors Chloride Channels / metabolism Membrane Potentials Paramecium tetraurelia* / genetics Paramecium tetraurelia* / metabolism Protozoan Proteins* / genetics Protozoan Proteins* / metabolism |
| IF | 2.143 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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