Reference - Detail
| RRC ID | 85463 |
|---|---|
| Author | Ozaki-Noma R, Wazawa T, Kakizuka T, Shidara H, Takemoto K, Nagai T. |
| Title | Positive-Type Reversibly Photoswitching Red Fluorescent Protein for Dual-Color Superresolution Imaging with Single Light Exposure for Off-Switching. |
| Journal | ACS Nano |
| Abstract |
Positive-type reversibly photoswitching fluorescent proteins (p-rsFPs) transition to a bright on-state upon light exposure for fluorescence excitation and to a dark off-state under a different wavelength. p-rsFPs are widely used in superresolution (SR) imaging techniques, offering simplified observation procedure and enhanced biocompatibility. Although some green p-rsFPs possess adequate photoproperties for SR imaging, all red p-rsFPs (p-rsRFPs) to date exhibit suboptimal properties, limiting the color palette for multiplexed SR imaging. Here, we present a p-rsRFP, rsZACRO, with 3.0-fold brighter fluorescence, 5.3-fold faster off-switching, and 1.5-fold higher on/off contrast than rsCherry, a conventional representative p-rsRFP. Using rsZACRO with superresolution polarization demodulation/on-state polarization angle narrowing (SPoD-OnSPAN), we successfully demonstrated SR imaging in the red spectrum and dual-color SR imaging with a single light for off-switching, visualizing vimentin intermediate filaments and actin filaments at higher spatial resolution than the diffraction limit of light in a living mammalian cell. |
| Volume | 19(7) |
| Pages | 7188-7201 |
| Published | 2025-2-25 |
| DOI | 10.1021/acsnano.4c16847 |
| PMID | 39937184 |
| PMC | PMC11867007 |
| MeSH | Animals Color Humans Light* Luminescent Proteins* / chemistry Optical Imaging* / methods Red Fluorescent Protein |
| IF | 14.588 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | rsZACRO/pcDNA3 (RDB20976) rsZACRO/pRSETB (RDB20977) |