| RRC ID |
85487
|
| 著者 |
Liu K, Chen M, Chang J, Yang J, Wang D, Ding X, Yang M, Zhang Y, Lu R, Chen L.
|
| タイトル |
H-NS represses fliD transcription for lateral flagellar filament cap in Vibrio parahaemolyticus.
|
| ジャーナル |
Arch Microbiol
|
| Abstract |
Vibrio parahaemolyticus propels itself through liquids using a polar flagellum and efficiently swarms across surfaces or viscous environments with the aid of lateral flagella. H-NS plays a negative role in the swarming motility of V. parahaemolyticus by directly repressing the transcription of the lateral flagellin gene lafA. However, it remains unknown whether H-NS can directly regulate other lateral flagellar genes in V. parahaemolyticus. In this study, we investigated the regulation of fliD, which is responsible for the lateral flagellar filament cap, by H-NS using quantitative real-time PCR, luminescence assay, two-plasmid lacZ fusion assay, electrophoretic mobility shift assay, and DNase I footprinting assay. We found that H-NS represses the transcription of fliD. Overexpression of H-NS in Escherichia coli resulted in the repression of the promoter activity of fliD. Furthermore, His-tagged H-NS was shown to protect a specific DNA region located 11 to 188 base pairs upstream of fliD from DNase I digestion. Therefore, it can be concluded that H-NS directly and negatively regulates the transcription of fliD. This study has contributed to our understanding of the regulatory mechanisms of H-NS on lateral flagella-propelled swarming motility in V. parahaemolyticus.
|
| 巻・号 |
207(2)
|
| ページ |
29
|
| 公開日 |
2025-1-9
|
| DOI |
10.1007/s00203-024-04217-8
|
| PII |
10.1007/s00203-024-04217-8
|
| PMID |
39786595
|
| MeSH |
Bacterial Proteins* / genetics
Bacterial Proteins* / metabolism
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Escherichia coli / genetics
Escherichia coli / metabolism
Flagella* / genetics
Flagella* / metabolism
Flagellin / genetics
Flagellin / metabolism
Gene Expression Regulation, Bacterial*
Promoter Regions, Genetic*
Transcription, Genetic*
Vibrio parahaemolyticus* / genetics
Vibrio parahaemolyticus* / metabolism
|
| IF |
1.884
|
| リソース情報 |
| 病原細菌 |
Vibrio parahaemolyticus |
