| RRC ID |
85552
|
| Author |
Fujie Y, Fusaki N, Katayama T, Hamasaki M, Soejima Y, Soga M, Ban H, Hasegawa M, Yamashita S, Kimura S, Suzuki S, Matsuzawa T, Akari H, Era T.
|
| Title |
New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood.
|
| Journal |
PLoS One
|
| Abstract |
Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into the cellular DNA, and can be easily eliminated. TS12KOS carries KLF4, OCT3/4, and SOX2 in a single vector and can easily generate iPSCs from human blood cells. Using TS12KOS, we established iPSC lines from chimpanzee blood, and used DNA array analysis to show that the global gene-expression pattern of chimpanzee iPSCs is similar to those of human embryonic stem cell and iPSC lines. These results demonstrated that our new vector is useful for generating iPSCs from the blood cells of both human and chimpanzee. In addition, the chimpanzee iPSCs are expected to facilitate unique studies into human physiology and disease.
|
| Volume |
9(12)
|
| Pages |
e113052
|
| Published |
2014-1-1
|
| DOI |
10.1371/journal.pone.0113052
|
| PII |
PONE-D-14-29786
|
| PMID |
25479600
|
| PMC |
PMC4257541
|
| MeSH |
Animals
Cell Differentiation / genetics*
Cell Line
Cellular Reprogramming / genetics
Gene Expression
Genetic Vectors*
Humans
Induced Pluripotent Stem Cells / cytology
Kruppel-Like Factor 4
Pan troglodytes
Sendai virus / genetics*
Transduction, Genetic*
Transgenes
|
| IF |
2.74
|
| Resource |
| GAIN |
id=339
id=334 |
