論文 - 詳細
| RRC ID | 85575 |
|---|---|
| 著者 | Hawley J, Lea R, Biga V, Papalopulu N, Manning C. |
| タイトル | Cellular signalling protrusions enable dynamic distant contacts in spinal cord neurogenesis. |
| ジャーナル | Biol Open |
| Abstract |
In the developing mouse ventral spinal cord, HES5, a transcription factor downstream of Notch signalling, is expressed as evenly spaced clusters of high HES5-expressing neural progenitor cells along the dorsoventral axis. While Notch signalling requires direct membrane contact for its activation, we have previously shown mathematically that contact needs to extend beyond neighbouring cells for the HES5 pattern to emerge. However, the presence of cellular structures that could enable such long-distance signalling was unclear. Here, we report that cellular protrusions are present all along the apicobasal axis of individual neural progenitor cells. Through live imaging, we show that these protrusions dynamically extend and retract reaching lengths of up to ∼20 µm, enough to extend membrane contact beyond adjacent cells. The Notch ligand DLL1 was found to colocalise with protrusions, further supporting the idea that Notch signalling can be transduced at a distance. The effect of protrusions on the HES5 pattern was tested by reducing the density of protrusions using the CDC42 inhibitor ML141, leading to a tendency to decrease the distance between high HES5 cell clusters. However, this tendency was not significant and leaves an open question about their role in the fine-grained organisation of neurogenesis. |
| 巻・号 | 14(1) |
| 公開日 | 2025-1-15 |
| DOI | 10.1242/bio.061765 |
| PII | 365406 |
| PMID | 39835478 |
| PMC | PMC11789279 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Calcium-Binding Proteins Cell Communication Cell Surface Extensions* / metabolism Intercellular Signaling Peptides and Proteins / metabolism Mice Neural Stem Cells / cytology Neural Stem Cells / metabolism Neurogenesis* Receptors, Notch / metabolism Repressor Proteins / metabolism Signal Transduction* Spinal Cord* / cytology Spinal Cord* / embryology Spinal Cord* / metabolism |
| IF | 2.029 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC06008 |