論文 - 詳細
| RRC ID | 85658 |
|---|---|
| 著者 | Kobayashi T, Suzuki T, Kawahara R, Harai N, Dohmae N, Simizu S. |
| タイトル | C-mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells. |
| ジャーナル | FEBS Lett |
| Abstract |
C-mannosylation is a protein glycosylation that regulates the functions of target proteins. Although it has been reported that a disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1), an important spermatogenesis factor, is C-mannosylated, the roles of C-mannosylation in ADAMTS1 in testicular cells are still unclear. In this study, we found that ADAMTS1 is C-mannosylated at Trp562 and Trp565 in testis germ NEC8 cells. To determine the roles of C-mannosylation in ADAMTS1, we established cells expressing a C-mannosylation-defective ADAMTS1, in which C-mannosylated tryptophan residues were replaced with phenylalanine residues (ADAMTS1/2WF). Processing and secretion of ADAMTS1/2WF were both inhibited compared to those of wild-type. Moreover, wild-type ADAMTS1 degraded aggrecan, whereas ADAMTS1/2WF could not. These results indicate the impact of C-mannosylation on ADAMTS1 function. |
| 公開日 | 2025-8-6 |
| DOI | 10.1002/1873-3468.70133 |
| PMID | 40878842 |
| PMC | PMC12599623 |
| MeSH | ADAMTS1 Protein* / genetics ADAMTS1 Protein* / metabolism Aggrecans / metabolism Cell Line, Tumor Glycosylation Humans Male Testicular Neoplasms* / genetics Testicular Neoplasms* / metabolism Testicular Neoplasms* / pathology |
| IF | 3.057 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NEC8(RCB0489) |
| 遺伝子材料 | CSII-CMV-ADAMTS1-MH-IRES2-Bsd (RDB21256) CSII-CMV-ADAMTS1/2WF-MH-IRES2-Bsd (RDB21257) |