| RRC ID |
85662
|
| 著者 |
Eiko Himi, Shiho Kurihara-Yonemoto, Fumitaka Abe, Hidekazu Takahashi, Keisuke Tanaka, Takakazu Matsuura, Masahiko Maekawa, Takuji Sasaki, Kazuhide Rikiishi
|
| タイトル |
Tamyb10-D1 restores red grain color and increases grain dormancy via suppressing expression of TaLTP2.128, non-specific lipid transfer protein in wheat
|
| ジャーナル |
Euphytica
|
| Abstract |
Grain dormancy of wheat is closely associated with grain color: red-grained lines show higher dormancy than white-grained lines. The production of red pigments is regulated by R-1, Tamyb10 gene. However, the relation between grain color and dormancy remains unknown. For this study, we generated transgenic lines which were introduced a DNA fragment containing Tamyb10-D1 gene and its a 2 kb promoter including the 5′ untranslated region into white-grained wheat. Transgenic lines showed red-grained and higher dormant traits. Contents of plant hormones and gene expression of embryos at 30 days after pollination were examined in a wild type and a transgenic line. No differences were observed in the contents of plant hormones, but several genes are differentially expressed between these lines. One differentially expressed gene, TaLTP2.128, is a member of non-specific lipid transfer proteins. It was expressed higher in white grains than in red grains. A putative amino acid sequence showed similarity to that of OsHyPRP5, which is identified as QTL controlling low-temperature germinability in rice. Expression of TaLTP2.128 was increased by grain imbibition. The increasing levels were higher not only in other white-grained lines, but also in non-dormant red-grained lines. TaLTP2.128 was expressed at a quite early stage of germination. These study findings indicate that Tamyb10 regulates dormancy release by the modification of TaLTP2.128 acting as trigger of germination.
|
| 巻・号 |
220
|
| 公開日 |
2024-2-1
|
| DOI |
10.1007/s10681-023-03265-3
|
| IF |
1.614
|
| リソース情報 |
| コムギ |
KT020-061 cv. Fielder |
