論文 - 詳細
| RRC ID | 85710 |
|---|---|
| 著者 | Cao Y, Zhou Z, He S, Liu W. |
| タイトル | TTYH3 promotes the malignant progression of oral squamous cell carcinoma SCC-9 cells by regulating tumor-associated macrophage polarization. |
| ジャーナル | Arch Oral Biol |
| Abstract |
OBJECTIVE:This study was designed to investigate the biological role and the reaction mechanism of Tweety family member 3 (TTYH3) in oral squamous cell carcinoma (OSCC). DESIGN:The mRNA and protein expressions of TTYH3 were assessed with RT-qPCR and western blot. After silencing TTYH3 expression, the proliferation of OSCC cells were detected using cell counting kit-8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridine (EdU) staining and colony formation assay. Cell migration and invasion were detected using wound healing and transwell. Gelatin zymography protease assay was used to detect matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-2 (MMP9) activity and western blot was used to detect the expressions of proteins associated with proliferation and epithelial-mesenchymal transition (EMT). The mRNA expression of TTYH3 in THP-1-derived macrophage was detected using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). The number of CD86-positive cells and CD206-positive cells was detected using immunofluorescence assay. RT-qPCR was used to detect the expressions of M2 markers arginase 1 (ARG1), chitinase-like 3 (YM1) and mannose receptor C-type 1 (MRC1). RESULTS:In this study, it was found that TTYH3 expression was upregulated in OSCC cell lines and TTYH3 knockdown could inhibit the proliferation, migration, invasion and EMT process in OSCC via suppressing M2 polarization of tumor-associated macrophages. CONCLUSIONS:Collectively, TTYH3 facilitated the progression of OSCC through the regulation of tumor-associated macrophages polarization. |
| 巻・号 | 165 |
| ページ | 106028 |
| 公開日 | 2024-9-1 |
| DOI | 10.1016/j.archoralbio.2024.106028 |
| PII | S0003-9969(24)00149-3 |
| PMID | 38908074 |
| MeSH | Blotting, Western Carcinoma, Squamous Cell* / genetics Carcinoma, Squamous Cell* / metabolism Carcinoma, Squamous Cell* / pathology Cell Line, Tumor Cell Movement* Cell Proliferation* Disease Progression Epithelial-Mesenchymal Transition* Humans Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinase 9 / metabolism Mouth Neoplasms* / genetics Mouth Neoplasms* / metabolism Mouth Neoplasms* / pathology Real-Time Polymerase Chain Reaction Tumor-Associated Macrophages* / metabolism |
| IF | 1.931 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-2(RCB1945) HSC-3(RCB1975) |