論文 - 詳細
| RRC ID | 85739 |
|---|---|
| 著者 | Brown ST, Holla MR, Land MA, Yang S, McDonald AJ, St-Pierre F, Raman IM. |
| タイトル | High-speed voltage imaging of action potentials in molecular layer interneurons reveals sensory-driven synchrony that augments movement. |
| ジャーナル | Cell Rep |
| Abstract |
Testing whether the synchrony of action potential firing is a cerebellar coding mechanism requires simultaneous recording, with high temporal fidelity, from populations of identified neurons. Here, we used targeted one-photon voltage imaging at 2-4 kHz to record action potentials from groups of ∼10-300 molecular layer interneurons (MLIs) expressing a positively tuned, genetically encoded voltage indicator, FORCE1f or pAce. In awake resting mice, crus I MLIs fired brief (∼1-ms) spikes at 20-60 spikes/s. Sensory stimuli of air puffs to the whiskers evoked short-latency (<10 ms) increases in spiking probability. In most trials, >50% of MLIs fired synchronously with 4-ms temporal precision. The magnitude of puff-evoked whisks correlated tightly with the trial-by-trial percentage of synchrony. Brief optogenetic stimulation of MLIs was sufficient to induce and augment whisker protraction, whereas overriding MLI inhibition by stimulating target Purkinje cells reduced protractions, providing direct evidence that sensory-evoked spike synchrony can generate movement. |
| 巻・号 | 44(8) |
| ページ | 116148 |
| 公開日 | 2025-8-26 |
| DOI | 10.1016/j.celrep.2025.116148 |
| PII | S2211-1247(25)00919-2 |
| PMID | 40811060 |
| MeSH | Action Potentials* / physiology Animals Interneurons* / physiology Male Mice Mice, Inbred C57BL Movement / physiology Optogenetics Purkinje Cells / physiology Vibrissae / physiology |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 23 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 1.5 |
| リソース情報 | |
| 実験動物マウス | RBRC10109 |