| RRC ID |
85748
|
| Author |
Ikari A, Ito Y, Taniguchi K, Shibata MA, Kimura K, Iwamoto M, Lee SW.
|
| Title |
Role of CD44-Positive Extracellular Vesicles Derived from Highly Metastatic Mouse Mammary Carcinoma Cells in Pre-Metastatic Niche Formation.
|
| Journal |
Int J Mol Sci
|
| Abstract |
Malignant breast cancers pose a notable challenge when it comes to treatment options. Recently, research has implicated extracellular vesicles (EVs) secreted by cancer cells in the formation of a pre-metastatic niche. Small clumps of CD44-positive breast cancer cells are efficiently transferred through CD44-CD44 protein homophilic interaction. This study aims to examine the function of CD44-positive EVs in pre-metastatic niche formation in vitro and to suggest a more efficacious EV formulation. We used mouse mammary carcinoma cells, BJMC3879 Luc2 (Luc2 cells) as the source of CD44-positive EVs and mouse endothelial cells (UV2 cells) as the recipient cells in the niche. Luc2 cells exhibited an enhanced secretion of EVs expressing CD44 and endothelial growth factors (VEGF-A, -C) under 20% O2 (representative of the early stage of tumorigenesis) compared to its expression under 1% O2 (in solid tumor), indicating that pre-metastatic niche formation occurs in the early stage. Furthermore, UV2 endothelial cells expressing CD44 demonstrated a high level of engulfment of EVs that had been supplemented with hyaluronan, and the proliferation of UV2 cells occurred following the engulfment of EVs. These results suggest that anti-VEGF-A and -C encapsulated, CD44-expressing, and hyaluronan-coated EVs are more effective for tumor metastasis.
|
| Volume |
25(17)
|
| Published |
2024-9-9
|
| DOI |
10.3390/ijms25179742
|
| PII |
ijms25179742
|
| PMID |
39273689
|
| PMC |
PMC11395953
|
| MeSH |
Animals
Cell Line, Tumor
Cell Proliferation
Endothelial Cells / metabolism
Endothelial Cells / pathology
Extracellular Vesicles* / metabolism
Female
Hyaluronan Receptors* / metabolism
Hyaluronic Acid / metabolism
Mammary Neoplasms, Animal / metabolism
Mammary Neoplasms, Animal / pathology
Mice
Neoplasm Metastasis
Tumor Microenvironment
Vascular Endothelial Growth Factor A / metabolism
|
| IF |
4.556
|
| Resource |
| Human and Animal Cells |
UV♀2(RCB1994) |
