論文 - 詳細
| RRC ID | 85768 |
|---|---|
| 著者 | Suzuki M, Kawauchi K, Machiyama H, Hirata H, Ishiwata S, Fujita H. |
| タイトル | Dynamic Remodeling of Mechano-Sensing Complexes in Suspended Fibroblast Cell-Sheets Under External Mechanical Stimulus. |
| ジャーナル | Biotechnol Bioeng |
| Abstract |
Freestanding cell-sheets are valuable bio-materials for use in regenerative medicine and tissue engineering. Because cell-sheets experience various mechanical stimulations during handling, it is important to understand the responses of cells to these stimulations. Here, we demonstrate changes in the localization of various proteins during the stretching of fibroblast cell-sheets. These proteins are known to be involved in mechano-sensing. Upon stretching, actin filaments appear parallel to the stretching direction. At cell-cell junctions, β-catenin forms clusters that co-localize with accumulated vinculin and zyxin as well as the actin filaments. The p130 Crk-associated substrate, known to be present in focal adhesions, is also recruited to these clusters and phosphorylated. Our results suggest that mechano-sensing machinery is formed at cell-cell junctions when the cell-sheets are stretched. |
| 巻・号 | 122(7) |
| ページ | 1929-1940 |
| 公開日 | 2025-7-1 |
| DOI | 10.1002/bit.28996 |
| PMID | 40270085 |
| PMC | PMC12152469 |
| MeSH | Animals Fibroblasts* / cytology Fibroblasts* / metabolism Fibroblasts* / physiology Intercellular Junctions / metabolism Mechanotransduction, Cellular* / physiology Mice Stress, Mechanical Vinculin / metabolism Zyxin / metabolism beta Catenin / metabolism |
| IF | 4.002 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH 3T3 |