Reference - Detail
|Author||Hara H, Narita S, Karibian D, Park JT, Yamamoto Y, Nishimura Y.|
|Title||Identification and characterization of the Escherichia coli envC gene encoding a periplasmic coiled-coil protein with putative peptidase activity.|
|Journal||FEMS Microbiol. Lett.|
PM61 is a chain-forming envC strain of Escherichia coli with a leaky outer membrane. It was found to have an oversized penicillin-binding protein 3, which was the result of an IS4 insertion in the prc gene. The other properties of PM61 were caused by the envC mutation. We cloned the envC (yibP) gene and identified the mutation site, causing a single residue substitution, H366Y, in the PM61 envC allele. The gene product was predicted to be a periplasmic protein having coiled-coil structure in the N-terminal region and homology to lysostaphin in the C-terminal region. Overexpression of envC inhibited cell growth, and overexpression of the PM61 mutant allele caused cell lysis. Disruption of the chromosomal envC caused the same defects as the envC point mutation, indicating the gene is dispensable for growth but important for normal septation/separation and cell envelope integrity.
|MeSH||Alleles Amino Acid Substitution / genetics Bacterial Outer Membrane Proteins / chemistry Bacterial Outer Membrane Proteins / genetics Bacterial Outer Membrane Proteins / metabolism Bacterial Proteins / chemistry* Bacterial Proteins / genetics* Bacterial Proteins / metabolism Carrier Proteins* Cell Membrane / chemistry Chromosomes, Bacterial Cloning, Molecular Escherichia coli / genetics* Escherichia coli Proteins* Gene Expression Regulation, Bacterial Hexosyltransferases / genetics Membrane Proteins / chemistry* Membrane Proteins / genetics* Membrane Proteins / metabolism Multidrug Resistance-Associated Proteins Multienzyme Complexes / genetics Muramoylpentapeptide Carboxypeptidase* Mutagenesis, Insertional Penicillin-Binding Proteins Peptide Hydrolases / metabolism Peptidoglycan Glycosyltransferase* Peptidyl Transferases / genetics Point Mutation / genetics* Protein Conformation|
|Prokaryotes E. coli||JE7935 JE7987 JE7988 JE7989 JE7990 JE7991 JE7992 JE7994|