RRC ID 85882
Author Aoki K, Yoshitani N, Kurio N, Yoshioka N, Teramachi J, Ikegame M, Okamura H, Ibaragi S.
Title Angiogenin-induced Osteoclastogenesis Mediates Bone Destruction in Oral Squamous Carcinoma.
Journal Anticancer Res
Abstract BACKGROUND/AIM:Bone destruction caused by oral cancer severely impacts patient quality of life. This study aimed to clarify the role of angiogenin (ANG) in osteoclastogenesis and oral cancer-induced bone destruction.
MATERIALS AND METHODS:Recombinant ANG was used to assess its effects on osteoclast formation and bone resorption activity in bone marrow cultures. ANG-knockdown oral squamous carcinoma HSC-2 cells (ANG-RNAi) were transplanted into intramedullary cavities of femurs. Bone destruction was radiologically analyzed, while angiogenesis and osteoclast induction in the surrounding area of the transplanted lesion were histologically examined.
RESULTS:Recombinant ANG promoted osteoclast formation and bone resorption activity. Transplantation of ANG-RNAi cells significantly reduced tumor growth and bone destruction properties compared to transplantation of control cells. Histological analysis revealed lower angiogenesis and fewer osteoclast induction in the ANG-RNAi cells-transplanted group.
CONCLUSION:ANG mediates oral cancer-induced bone destruction by promoting osteoclast formation and resorption. These findings suggest that ANG could be a potential therapeutic target for suppressing tumor growth, angiogenesis, and bone destruction in oral cancer therapy.
Volume 45(3)
Pages 1025-1033
Published 2025-3-1
DOI 10.21873/anticanres.17489
PII 45/3/1025
PMID 40037849
MeSH Animals Bone Resorption* / metabolism Bone Resorption* / pathology Carcinoma, Squamous Cell* / genetics Carcinoma, Squamous Cell* / metabolism Carcinoma, Squamous Cell* / pathology Cell Line, Tumor Humans Male Mice Mouth Neoplasms* / genetics Mouth Neoplasms* / metabolism Mouth Neoplasms* / pathology Neovascularization, Pathologic / pathology Osteoclasts* / drug effects Osteoclasts* / metabolism Osteoclasts* / pathology Osteogenesis* / drug effects Ribonuclease, Pancreatic* / genetics Ribonuclease, Pancreatic* / metabolism
IF 1.994
Resource
Human and Animal Cells HSC-2(RCB1945)