Reference - Detail
| RRC ID | 85887 |
|---|---|
| Author | Nakagawa T, Emori C, Ikawa M. |
| Title | Trophectoderm-specific gene manipulation using adeno-associated viral vectors. |
| Journal | Exp Anim |
| Abstract |
In mammals, blastocyst-stage trophectoderm (TE) contacts the maternal body at the time of implantation and forms the placenta after implantation, which supports the development of the fetus. Studying gene function in TE and placenta is important to understand normal implantation and pregnancy processes and their dysfunction. However, genetically modified mice are commonly generated by manipulating pronuclear-stage zygotes, which modify both the genome of the fetus and the placenta. Therefore, we previously developed TE/placenta-specific gene expression technology by transducing blastocysts with lentiviral vectors. However, the zona pellucida (ZP) needed to be removed before transduction. In this study, we examined various adeno-associated viral (AAV) vectors to develop a new TE/placenta-specific gene transduction method. As AAV1 can path through ZP, we succeeded in trophoblast-specific gene expression without ZP removal. Furthermore, TE cells genetically modified by AAV1-Cre contributed uniformly to the placenta. Our new technology contributes to advances in implantation and placenta research and leads to the development of new assisted reproductive technology. |
| Volume | 74(3) |
| Pages | 310-318 |
| Published | 2025-7-11 |
| DOI | 10.1538/expanim.24-0165 |
| PMID | 39805595 |
| PMC | PMC12270592 |
| MeSH | Animals Blastocyst / metabolism Dependovirus* / genetics Embryo Implantation / genetics Female Gene Expression Genetic Vectors* / genetics Mice Placenta / metabolism Pregnancy Transduction, Genetic* / methods Trophoblasts* / metabolism Zona Pellucida / metabolism |
| IF | 1.574 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pscAAV-CAG-Cre (RDB20979) |