Reference - Detail
| RRC ID | 85888 |
|---|---|
| Author | Sahoo BR, Kocman V, Clark N, Myers N, Deng X, Wong EL, Yang HJ, Kotar A, Guzman BB, Dominguez D, Plavec J, Bardwell JCA. |
| Title | Protein G-quadruplex interactions and their effects on phase transitions and protein aggregation. |
| Journal | Nucleic Acids Res |
| Abstract |
The SERF family of proteins were originally discovered for their ability to accelerate amyloid formation. Znf706 is an uncharacterized protein whose N-terminus is homologous to SERF proteins. We show here that human Znf706 can promote protein aggregation and amyloid formation. Unexpectedly, Znf706 specifically interacts with stable, non-canonical nucleic acid structures known as G-quadruplexes. G-quadruplexes can affect gene regulation and suppress protein aggregation; however, it is unknown if and how these two activities are linked. We find Znf706 binds preferentially to parallel G-quadruplexes with low micromolar affinity, primarily using its N-terminus, and upon interaction, its dynamics are constrained. G-quadruplex binding suppresses Znf706's ability to promote protein aggregation. Znf706 in conjunction with G-quadruplexes therefore may play a role in regulating protein folding. RNAseq analysis shows that Znf706 depletion specifically impacts the mRNA abundance of genes that are predicted to contain high G-quadruplex density. Our studies give insight into how proteins and G-quadruplexes interact, and how these interactions affect both partners and lead to the modulation of protein aggregation and cellular mRNA levels. These observations suggest that the SERF family of proteins, in conjunction with G-quadruplexes, may have a broader role in regulating protein folding and gene expression than previously appreciated. |
| Volume | 52(8) |
| Pages | 4702-4722 |
| Published | 2024-5-8 |
| DOI | 10.1093/nar/gkae229 |
| PII | 7640530 |
| PMID | 38572746 |
| PMC | PMC11077067 |
| MeSH | Amyloid / chemistry Amyloid / genetics Amyloid / metabolism DNA-Binding Proteins* / chemistry DNA-Binding Proteins* / metabolism G-Quadruplexes* Humans Phase Transition Protein Aggregates* Protein Binding RNA, Messenger / chemistry RNA, Messenger / genetics RNA, Messenger / metabolism |
| IF | 11.502 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pET-HisSumo-hZnf706 (RDB20982) |