論文 - 詳細
| RRC ID | 85942 |
|---|---|
| 著者 | Seino R, Nishikubo K, Fukunaga H. |
| タイトル | Cell cycle- and dose-dependent effects on mitochondrial DNA copy number variation following irradiation. |
| ジャーナル | J Cell Sci |
| Abstract |
Cell survival after irradiation depends on the cell cycle at the time of exposure. This has been thought to be due to cell cycle-dependent nuclear DNA damage repair mechanisms. Here, we show the relationships between the exposed dose, the cell cycle phase at the time of exposure and changes in mitochondrial DNA copy numbers (mtDNAcn) after irradiation. We used a fluorescent ubiquitylation-based cell cycle indicator (FUCCI), which allows visualization of the cell cycle, and confirmed cell cycle synchronization in human cervical HeLa cells. In synchronous HeLa-FUCCI cells, the mtDNAcn changed with the progression of the cell cycle. Also, G1 phase-synchronized cells showed a dose-dependent increase of mtDNAcn at 48 h after X-ray exposure, whereas G2 cells showed a dose-dependent increase at 24 h. In addition, S phase-synchronized cells showed a dose-dependent increase at 24 and 48 h after irradiation. These results showed the cell cycle- and dose-dependent effects on mtDNAcn after irradiation, which might shed light on the emerging role of mitochondrial genome and in cell survival. |
| 巻・号 | 138(15) |
| 公開日 | 2025-8-1 |
| DOI | 10.1242/jcs.263642 |
| PII | 368851 |
| PMID | 40679155 |
| PMC | PMC12377708 |
| MeSH | Cell Cycle* / genetics Cell Cycle* / radiation effects Cell Survival / radiation effects DNA Copy Number Variations* / genetics DNA Copy Number Variations* / radiation effects DNA, Mitochondrial* / genetics DNA, Mitochondrial* / radiation effects Dose-Response Relationship, Radiation HeLa Cells Humans Mitochondria / genetics Mitochondria / radiation effects X-Rays |
| IF | 4.573 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa/Fucci(RCB2812) |