| RRC ID |
85999
|
| Author |
Aoyama K, Takazaki H, Arie M, Suemune H, Kawai S.
|
| Title |
Cryo-STEM Tomography for Cell biology Using Thick Lamella.
|
| Journal |
Microscopy (Oxf)
|
| Abstract |
Electron tomography (ET) is a powerful tool for structural studies in cell biology, but specimen thickness remains a significant limitation. Scanning transmission electron microscopy (STEM) tomography offers advantages in this regard. Recent developments in focused ion beam (FIB) slicing methods for cryo-cell biology have enabled the observation and 3D reconstruction of relatively thick specimens (300-500 nm) using cryo-STEM tomography. Organelles such as mitochondria and the nuclear membrane have been clearly reconstructed, demonstrating the promise of STEM tomography for structural studies in cell biology.
|
| Published |
2025-3-4
|
| DOI |
10.1093/jmicro/dfaf017
|
| PII |
8051765
|
| PMID |
40037755
|
| IF |
1.394
|
| Resource |
| Yeast |
|
