RRC ID 860
著者 Shiba Y, Yokoyama Y, Aono Y, Kiuchi T, Kusaka J, Matsumoto K, Hara H.
タイトル Activation of the Rcs signal transduction system is responsible for the thermosensitive growth defect of an Escherichia coli mutant lacking phosphatidylglycerol and cardiolipin.
ジャーナル J Bacteriol
Abstract The lethal effect of an Escherichia coli pgsA null mutation, which causes a complete lack of the major acidic phospholipids, phosphatidylglycerol and cardiolipin, is alleviated by a lack of the major outer membrane lipoprotein encoded by the lpp gene, but an lpp pgsA strain shows a thermosensitive growth defect. Using transposon mutagenesis, we found that this thermosensitivity was suppressed by disruption of the rcsC, rcsF, and yojN genes, which code for a sensor kinase, accessory positive factor, and phosphotransmitter, respectively, of the Rcs phosphorelay signal transduction system initially identified as regulating the capsular polysaccharide synthesis (cps) genes. Disruption of the rcsB gene coding for the response regulator of the system also suppressed the thermosensitivity, whereas disruption of cpsE did not. By monitoring the expression of a cpsB'-lac fusion, we showed that the Rcs system is activated in the pgsA mutant and is reverted to a wild-type level by the rcs mutations. These results indicate that envelope stress due to an acidic phospholipid deficiency activates the Rcs phosphorelay system and thereby causes the thermosensitive growth defect independent of the activation of capsule synthesis.
巻・号 186(19)
ページ 6526-35
公開日 2004-10-1
DOI 10.1128/JB.186.19.6526-6535.2004
PII 186/19/6526
PMID 15375134
PMC PMC516613
MeSH Bacterial Proteins / physiology* Cardiolipins / physiology* Escherichia coli / growth & development* Escherichia coli / metabolism Escherichia coli Proteins / physiology* Multienzyme Complexes / physiology* Phosphatidylglycerols / physiology* Phosphoprotein Phosphatases / physiology* Protein Kinases / physiology* Signal Transduction / physiology* Temperature Transcription Factors* Transferases (Other Substituted Phosphate Groups) / physiology*
IF 3.006
引用数 67
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME8454(SG20043)
情報 E.coli