RRC ID 86068
著者 Kumar S, Koseki Y, Tanita K, Shibata A, Mizutani A, Kasai H.
タイトル SN-38-indoximod conjugate: carrier free nano-prodrug for cancer therapy.
ジャーナル Ther Deliv
Abstract BACKGROUND:The integration of immunotherapy alongside chemotherapy represents a crucial approach in the treatment of cancer. Herein we report the SN-38-indoximod conjugate nano-prodrug to address the difficulties encountered by individuals. In this prodrug, SN-38 is connected to indoximod through a specific disulfide linker, which enables the release of the components in response to the tumor microenvironment characterized by elevated levels of glutathione, thereby facilitating programmed chemoimmunotherapy.
RESULTS:SN-38-indoximod conjugate was synthesized and fabricated to nano-prodrug by reprecipitation method. It showed comparable anti-cancer activity against A549 cells than SN-38 (IC50 = 0.24 ± 0.01 µM) with IC50 value 0.32 ± 0.04 µM. It inhibited 90% A549 cell at very lower concentration (IC90 = 6.07 ± 0.41 µM) as compared with SN-38 (IC90 = 24.60 ± 1.24 µM) and mixture of SN-38: indoximod (1:1, IC90 >30 µM). The nano-prodrug showed better size distribution profile and dispersion stability contains nanoparticles in effective size range (80-160 nm) required for the EPR effect.
CONCLUSION:This research offers valuable insights into the advancement of conjugate nano-prodrugs exhibiting synergistic pharmacological effects, while also presenting novel opportunities for the design of prodrug molecules capable of releasing drugs in response to diverse triggers.
巻・号 16(3)
ページ 217-226
公開日 2025-3-1
DOI 10.1080/20415990.2025.2458449
PMID 39887189
PMC PMC11875466
MeSH A549 Cells Antineoplastic Agents* / administration & dosage Antineoplastic Agents* / chemistry Antineoplastic Agents* / pharmacology Cell Line, Tumor Cell Survival / drug effects Drug Carriers / chemistry Drug Liberation Humans Nanoparticles* / chemistry Particle Size Prodrugs* / administration & dosage Prodrugs* / chemistry Prodrugs* / pharmacology Tumor Microenvironment / drug effects
リソース情報
ヒト・動物細胞 A549(RCB3677) MCF7(RCB1904)