論文 - 詳細
| RRC ID | 86071 |
|---|---|
| 著者 | Tachibana K, Sugimura S, Sakimura S, Bai L, Aoyama H, Takeda H, Niwa Y, Nagahama M, Kondoh M. |
| タイトル | Size-selective permeation-enhancing modulation of the tight junction by receptor-binding domains of Clostridium perfringens enterotoxin and Clostridium perfringens iota-toxin. |
| ジャーナル | Tissue Barriers |
| Abstract |
Modulation of claudin-based bicellular tight junction (TJ) and angulin-based tricellular TJ seals has been shown to enhance mucosal permeation of macromolecules, by using the receptor-binding fragments of Clostridium perfringens enterotoxin (C-CPE194, C-CPEmt, and C-CPEm19) and Clostridium perfringens iota-toxin (angubindin-1) as claudin modulators and an angulin modulator, respectively. Here, we compared the activity of these modulators on the TJ in human intestinal Caco-2 cells. All the claudin modulators loosened TJ integrity more potently compared to angubindin-1 with the order of potency being C-CPEm19 > C-CPE194 > C-CPEmt, and results for permeation enhancement were similar. Treatment with C-CPEmt and C-CPE194 at 100 µg/mL for 48 h enhanced the permeation of dextran sized 20 kDa and 70 kDa, respectively. Treatment with C-CPEm19 at 30 µg/mL for 48 h enhanced permeation of dextran with a molecular mass of up to 150 kDa. Furthermore, co-treatment of bicellular TJ modulators, such as C-CPEmt, C-CPE194, and C-CPEm19, and tricellular TJ modulators, such as angubindin-1, showed additive TJ-loosening and permeation-enhancing activities compared with individual treatments; specifically, C-CPEm19 and angubindin-1 co-treatment increased permeation of large molecules (70 kDa and 150 kDa). These findings indicate that TJ modulators may be used as size-selective permeation enhancers. |
| 巻・号 | 13(3) |
| ページ | 2459963 |
| 公開日 | 2025-1-1 |
| DOI | 10.1080/21688370.2025.2459963 |
| PMID | 39910789 |
| PMC | PMC12506900 |
| MeSH | ADP Ribose Transferases* Bacterial Toxins* / chemistry Bacterial Toxins* / metabolism Bacterial Toxins* / pharmacology Caco-2 Cells Clostridium perfringens* / metabolism Enterotoxins* / chemistry Enterotoxins* / metabolism Enterotoxins* / pharmacology Humans Tight Junctions* / drug effects Tight Junctions* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |