論文 - 詳細
| RRC ID | 86130 |
|---|---|
| 著者 | Julian T, Tang T, Tanga N, Yang Y, Hosokawa Y, Yalikun Y. |
| タイトル | A long-term universal impedance flow cytometry platform empowered by adaptive channel height and real-time clogging-release strategy. |
| ジャーナル | Lab Chip |
| Abstract |
Impedance flow cytometry is a widely used label-free technique for single-cell analysis; however, its limited sensitivity and lack of universality have hindered its ability to replace conventional flow cytometry. In this study, we propose an adaptive microfluidic channel platform that dynamically adjusts the channel height to improve both measurement performance and system versatility. We found that reducing the channel height by one-third effectively decreases the distance between particles and the sensing electrodes, resulting in an average 3.2-fold amplification of the impedance signal. This approach also reduces signal variability by half, thereby enhancing measurement precision. Enhanced particle discrimination was demonstrated using a mixture of yeast cells and 6 μm beads, while robust cell phenotyping was achieved across multiple cell lines, including A549, C6, and NIH/3T3. By integrating this adaptive channel with an object detection algorithm, we successfully created a self-optimizing system that utilizes intentional, temporary clogging as a strategy to regulate channel height. These findings underscore the potential for a universal, high-performance impedance flow cytometry platform that simple, clog-resistant, and adaptable for a wide range of biomedical applications. |
| 巻・号 | 25(20) |
| ページ | 5268-5282 |
| 公開日 | 2025-10-7 |
| DOI | 10.1039/d5lc00673b |
| PMID | 40856636 |
| MeSH | Animals Electric Impedance Equipment Design Flow Cytometry* / instrumentation Flow Cytometry* / methods Humans Lab-On-A-Chip Devices* Mice Microfluidic Analytical Techniques* / instrumentation NIH 3T3 Cells |
| IF | 6.774 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) NIH/3T3(RCB2767) C6(RCB2854) |