Reference - Detail
| RRC ID | 86177 |
|---|---|
| Author | Sakamoto K, Yamamoto Y, Inaba H, Matsuura K. |
| Title | Strategy toward In-Cell Self-Assembly of an Artificial Viral Capsid from a Fluorescent Protein-Modified β-Annulus Peptide. |
| Journal | ACS Synth Biol |
| Abstract |
In-cell self-assembly of natural viral capsids is an event that can be visualized under transmission electron microscopy (TEM) observations. By mimicking the self-assembly of natural viral capsids, various artificial protein- and peptide-based nanocages were developed; however, few studies have reported the in-cell self-assembly of such nanocages. Our group developed a β-Annulus peptide that can form a nanocage called artificial viral capsid in vitro, but in-cell self-assembly of the capsid has not been achieved. Here, we designed an artificial viral capsid decorated with a fluorescent protein, StayGold, to visualize in-cell self-assembly. Fluorescence anisotropy measurements and fluorescence resonance energy transfer imaging, in addition to TEM observations of the cells and super-resolution microscopy, revealed that StayGold-conjugated β-Annulus peptides self-assembled into the StayGold-decorated artificial viral capsid in a cell. Using these techniques, we achieved the in-cell self-assembly of an artificial viral capsid. |
| Volume | 13(6) |
| Pages | 1842-1850 |
| Published | 2024-6-21 |
| DOI | 10.1021/acssynbio.4c00135 |
| PMID | 38729919 |
| MeSH | Capsid* / chemistry Capsid* / metabolism Capsid Proteins* / chemistry Capsid Proteins* / genetics Capsid Proteins* / metabolism Fluorescence Polarization Fluorescence Resonance Energy Transfer* Luminescent Proteins / chemistry Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Electron, Transmission Peptides* / chemistry Virus Assembly |
| IF | 4.411 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |