論文 - 詳細
| RRC ID | 86181 |
|---|---|
| 著者 | Nomura T, Kim JS, Iwata O, Yamada K, Atsuji K, Uehara-Yamaguchi Y, Yoshida T, Inoue K, Takahagi K, Sakurai T, Shinozaki K, Ito T, Suzuki K, Goda K, Mochida K. |
| タイトル | Genetic dissection of nonconventional introns reveals codominant noncanonical splicing code in Euglena. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Pre-mRNA splicing is essential for eukaryotic gene expression and is achieved through the accurate recognition of exon-intron boundaries. Although nonconventional introns, which do not follow the conventional GT-AG splicing rule, have been identified in several species, these introns are typically rare in any given genome. Here, we demonstrate the widespread occurrence of nonconventional introns (71.8% of all introns) in the Euglena agilis genome and identify consensus motifs at these nonconventional exon-intron boundaries. We assessed the splicing efficiency of nonconventional introns and variants with point mutations via genomic knock-in within the second exon of Glucan synthase-like 2 in Euglena gracilis and genetically defined the sequence signature (5'-N3CDG-/-CH'GN5-6|Rexon-3') required for their proper splicing. This signature is present in 61.2% of all nonconventional introns detected in the E. agilis genome. Accordingly, we present a noncanonical splicing code for Euglena introns, highlighting the global coexistence of dual splicing rules for conventional and nonconventional introns. |
| 巻・号 | 122(39) |
| ページ | e2509937122 |
| 公開日 | 2025-9-30 |
| DOI | 10.1073/pnas.2509937122 |
| PMID | 40986342 |
| PMC | PMC12501133 |
| MeSH | Euglena gracilis / genetics Euglenida* / genetics Exons / genetics Glucosyltransferases / genetics Introns* / genetics RNA Splicing* / genetics |
| IF | 9.412 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 9.2 |
| リソース情報 | |
| 藻類 | NIES-3945 |