| RRC ID |
86182
|
| 著者 |
Takahashi IY, Suzuki T, Hirooka S, Dohmae N, Miyagishima SY, Nagao R.
|
| タイトル |
Comparative analysis of biochemical and sequence features of PSI-LHCI supercomplexes from Cyanidium caldarium NIES-551 and Cyanidiococcus yangmingshanensis NIES-2137.
|
| ジャーナル |
Photosynth Res
|
| Abstract |
Cyanidiophyceae represent basal, extremophilic taxa subdivided into several genetically distinct orders. While photosystem I supercomplexes containing light-harvesting complexes (PSI-LHCI) have been characterized in Cyanidioschyzonales and Galdieriales, their organization in Cyanidiales remains poorly understood. Here, we purified the PSI-LHCI supercomplex from Cyanidium caldarium NIES-551, and analyzed its biochemical and spectroscopic properties. SDS-PAGE and mass spectrometry identified canonical PSI and LHCI subunits. The chloroplast-encoded PSI proteins were identical to those of a previously sequenced Cd. caldarium strain. Comparative sequence analysis revealed substantial divergence in both PSI and LHCI proteins between the NIES-551 strain and Cyanidiococcus yangmingshanensis NIES-2137, a strain recently reclassified from Cd. caldarium. Pigment profiling of PSI-LHCI showed similar species across strains, yet carotenoid-to-chlorophyll a ratios were lower in NIES-551. 77-K fluorescence-emission spectra of PSI-LHCI highlighted a red-shifted emission peak in NIES-551 (734 nm) relative to NIES-2137 (727 nm), suggesting differences in pigment configuration and excitation-energy transfer. These findings indicate that PSI-LHCI in NIES-551 possesses lineage-specific pigment composition and LHCI organization. Together, these characteristics likely represent hallmarks of PSI-LHCI in Cyanidiales. Our results reinforce the evolutionary distinctiveness of Cyanidiales within Cyanidiophyceae and provide a foundation for future structural studies that will clarify the diversification of photosynthetic architecture in early-diverging red algae.
|
| 巻・号 |
163(5)
|
| ページ |
48
|
| 公開日 |
2025-9-17
|
| DOI |
10.1007/s11120-025-01169-y
|
| PII |
10.1007/s11120-025-01169-y
|
| PMID |
40960655
|
| MeSH |
Carotenoids / metabolism
Light-Harvesting Protein Complexes* / chemistry
Light-Harvesting Protein Complexes* / genetics
Light-Harvesting Protein Complexes* / metabolism
Photosystem I Protein Complex* / chemistry
Photosystem I Protein Complex* / genetics
Photosystem I Protein Complex* / metabolism
Phylogeny
Rhodophyta* / genetics
Rhodophyta* / metabolism
|
| IF |
3.216
|
| リソース情報 |
| 藻類 |
NIES-551
NIES-2137 |
