論文 - 詳細
| RRC ID | 86198 |
|---|---|
| 著者 | Lu F, Cremers A, Poeze M, van Griensven M, Welting TJM, Blokhuis TJ, Caron MMJ. |
| タイトル | N1 and N2 neutrophil phenotypes may play differential roles in stimulating the endochondral ossification behaviors of ATDC5 cells. |
| ジャーナル | Gene |
| Abstract |
OBJECTIVE:Endochondral ossification is vital for bone healing, with neutrophils playing a crucial role in the osteoimmune system. While N1 (pro-inflammatory) and N2 (regenerative) neutrophils are documented in other contexts, their role in endochondral ossification remains unclear. This study investigates their effects on ATDC5 cells. METHODS:Neutrophils from five healthy volunteers were isolated and polarized into N0 (unstimulated), N1, or N2 phenotypes. After culturing for 4 h, neutrophil-conditioned media was mixed (20 % v/v) with chondrogenic differentiation media (DM). ATDC5 cells were cultured with mixture or DM alone for 24 h, followed by continued DM culturing. On days 7 and 14, several gene expressions, ALP activity, and TGF-β3 levels were assessed. RESULTS:SOX9 peaked in the ATDC5 (A)/N2 group on day 7, while the A/N1 group showed the highest levels on day 14. COL2A1 was elevated in the A/N0 group on day 7. RUNX2 was higher in A/N1 and A/N2 on day 7, with A/N1 remaining elevated on day 14. MMP-13 was significantly higher in A/N1 on day 7. COL10A1 expression showed no significant changes. COL1A1 and COX2 were continually elevated in the A/N1 group. ALP activity was consistently enhanced in the A/N1 group, and TGF-β3 was lower in both A/N1 and A/N2 on day 14. CONCLUSIONS:This study indicates that N1 neutrophils may promote chondrocyte maturation and osteogenic differentiation, while N2 neutrophils may support proliferation, hypertrophy, and maturation, providing a cell reservoir for ossification. These findings highlight their distinct roles in directing chondrogenic progenitors toward bone rather than cartilage formation. |
| 巻・号 | 976 |
| ページ | 149874 |
| 公開日 | 2025-11-4 |
| DOI | 10.1016/j.gene.2025.149874 |
| PII | S0378-1119(25)00663-8 |
| PMID | 41197999 |
| IF | 2.984 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |