| RRC ID |
86232
|
| 著者 |
Uematsu H, Shimizu S, Onuma K, Coppo R, Sato Y, Matsunaga T, Fujiwara Y, Inoue M.
|
| タイトル |
Interaction between gastric cancer organoids and peritoneal mesothelial cells suppresses the growth of gastric cancer organoids and induces drug resistance.
|
| ジャーナル |
Hum Cell
|
| Abstract |
The prognosis of gastric cancer with peritoneal dissemination is poor because of its resistance to chemotherapy. To investigate the mechanism of drug resistance in peritoneal metastasis, cancer organoids were established from the ascites of a patient with peritoneal metastases of gastric cancer. The histological characteristics of the tumors were preserved in the organoids. A co-culture system was established by overlaying human-derived mesothelial cells on gastric cancer organoids embedded in type IA collagen, mimicking peritoneal dissemination foci. When co-cultured with mesothelial cells, the proliferation of ascites-derived gastric cancer organoids and other primary gastric cancer organoids was suppressed. Soluble factors derived from mesothelial cells were involved in suppressing cell proliferation. Organoids in co-culture showed reduced sensitivity to paclitaxel. This co-culture model may provide a useful platform for studying drug resistance mechanisms in the microenvironment of gastric cancer peritoneal metastases.
|
| 巻・号 |
39(1)
|
| ページ |
8
|
| 公開日 |
2025-11-13
|
| DOI |
10.1007/s13577-025-01311-x
|
| PII |
10.1007/s13577-025-01311-x
|
| PMID |
41233623
|
| MeSH |
Ascites / pathology
Cell Proliferation
Coculture Techniques
Drug Resistance, Neoplasm* / genetics
Epithelium / pathology
Humans
Organoids* / pathology
Paclitaxel / pharmacology
Peritoneal Neoplasms* / drug therapy
Peritoneal Neoplasms* / pathology
Peritoneal Neoplasms* / secondary
Peritoneum* / cytology
Peritoneum* / pathology
Stomach Neoplasms* / drug therapy
Stomach Neoplasms* / genetics
Stomach Neoplasms* / pathology
Tumor Microenvironment
|
| IF |
3.463
|
| リソース情報 |
| ヒト・動物細胞 |
HOMC-B1(RCB5655) |
