Reference - Detail
| RRC ID | 86247 |
|---|---|
| Author | Osugi T, Shiraishi A, Sasakura Y, Sugahara K, Yamagaki T, Yamamoto T, Satake H. |
| Title | Two- and three-dimensional neuropeptidomic landscape in the central nervous system of an invertebrate chordate, Ciona robusta. |
| Journal | iScience |
| Abstract |
Neuroanatomical information on neuropeptidergic neurons provides crucial clues to understanding the functions of neuropeptides and the cerebral structure-function relationships. In this study, we constructed comprehensive two- and three-dimensional atlases of 23 neuropeptides in the central nervous system (CNS) of an invertebrate chordate, Ciona robusta, at the single-cell level using a mass spectrometry imaging workflow. The neuropeptidomic atlases revealed that the cerebral ganglion is divided into three functional areas, dorso-rostral, dorso-caudal, and ventral areas. Particularly, the distribution of Ciona tachykinin, cholecystokinin, and neurotensin-like-peptide demonstrated the basal cerebral structure-function relationships in reproductive regulation in Ciona. The atlases also demonstrated the unexpected colocalization of multiple neuropeptides and the differential localization of gene-related peptides yielded from a single precursor, which cannot be unraveled by any transcriptomic analyses. Collectively, the present study provides a foundation for investigating various neuropeptidergic functions of the invertebrate chordate CNS and the evolution of chordate neuropeptidergic systems. |
| Volume | 28(9) |
| Pages | 113413 |
| Published | 2025-9-19 |
| DOI | 10.1016/j.isci.2025.113413 |
| PII | S2589-0042(25)01674-8 |
| PMID | 40970208 |
| PMC | PMC12441681 |
| IF | 4.447 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.intestinalis / (O.japonicus) | Tg[MiCiPC2K]2 |