RRC ID 863
Author Haneda T, Okada N, Miki T, Danbara H.
Title Sequence analysis and characterization of sulfonamide resistance plasmid pRF-1 from Salmonella enterica serovar Choleraesuis.
Journal Plasmid
Abstract The nucleotide sequence of a small plasmid, designated pRF-1, isolated from Salmonella enterica serovar Choleraesuis, was determined. We identified seven open reading frames (ORFs) encoded by 6066 nucleotides with a total G + C content of 53.6%. Analysis of the complete nucleotide sequence revealed a replicon of pRF-1 to have high similarity to the p15A origin of replication, with a possible cer-like region. ORF1, which is composed of 816 nucleotides, shows a high degree of similarity to dihydropteroate synthetase encoded by the sulII gene from plasmids in several enteropathogenic bacteria, which functions as the sulfonamide resistance determinant. In fact, Salmonella and Escherichia coli strains carrying pRF-1 were found to show strong resistance to sulfathiazole, suggesting that orf1 is a functional gene. Four of seven ORFs were found to encode putative proteins of unknown function.
Volume 52(3)
Pages 218-24
Published 2004-11
DOI 10.1016/j.plasmid.2004.07.002
PII S0147-619X(04)00083-6
PMID 15518878
MeSH Base Sequence Drug Resistance, Bacterial / genetics* Plasmids / chemistry Plasmids / genetics* Restriction Mapping Salmonella enterica / drug effects Salmonella enterica / genetics* Sulfonamides / pharmacology*
IF 2.577
Times Cited 5
Prokaryotes E. coli ME7260(JC411)