| RRC ID |
86344
|
| Author |
Okahashi N, Shima T, Kondo Y, Araki C, Tsuji S, Sawai A, Uehara H, Kohno S, Shimizu H, Takahashi C, Matsuda F.
|
| Title |
Metabolic flux and flux balance analyses indicate the relevance of metabolic thermogenesis and aerobic glycolysis in cancer cells.
|
| Journal |
Metab Eng
|
| Abstract |
Adenosine triphosphate (ATP) regeneration by substrate-level phosphorylation is a general feature of cancer metabolism, even under normoxic conditions (aerobic glycolysis). However, it is unclear why cancer cells prefer inefficient aerobic glycolysis over the highly efficient process of oxidative phosphorylation for ATP regeneration. To investigate the metabolic principles underlying aerobic glycolysis, we performed 13C-metabolic flux analysis of 12 cultured cancer cell lines and explored the metabolic constraints required to reproduce the results using in silico metabolic simulations. We found that the measured flux distribution can be reproduced by maximizing the ATP consumption in the flux balance analysis considering a limitation of metabolic heat dissipation (enthalpy change). Consistent with the simulation, OXPHOS inhibition induced metabolic redirection to aerobic glycolysis while maintaining the intracellular temperature. Furthermore, the dependency on aerobic glycolysis was partly alleviated upon culturing at low temperatures. Our data suggest that metabolic thermogenesis is an important factor in understanding aerobic glycolysis in cancer cells and that an advantage of aerobic glycolysis is the reduction in metabolic heat generation during ATP regeneration.
|
| Volume |
92
|
| Pages |
185-193
|
| Published |
2025-11-1
|
| DOI |
10.1016/j.ymben.2025.08.002
|
| PII |
S1096-7176(25)00121-1
|
| PMID |
40783020
|
| MeSH |
Adenosine Triphosphate* / metabolism
Aerobiosis
Cell Line, Tumor
Glycolysis*
Humans
Metabolic Flux Analysis
Models, Biological*
Neoplasms* / metabolism
Neoplasms* / pathology
Oxidative Phosphorylation
Thermogenesis*
|
| IF |
7.263
|
| Resource |
| Human and Animal Cells |
HCT116(RCB2979)
MIA Paca-2(RCB2094)
PANC-1(RCB2095)
MCF7(RCB1904) |
