論文 - 詳細
| RRC ID | 86373 |
|---|---|
| 著者 | Oyama K, Ishii A, Matsumura S, Oyama TG, Taguchi M, Suzuki M. |
| タイトル | Spatiotemporal temperature control by holographic heating microscopy unveils cellular thermosensitive calcium signalling. |
| ジャーナル | Lab Chip |
| Abstract |
Optical microheating technologies have revealed how biological systems sense heating and cooling at the microscopic scale. Sensing is based on thermosensitive biochemical reactions that frequently engage membrane proteins, Ca2+ channels, and pumps to convert sensing information as the Ca2+ signalling in cells. These findings highlight the feasibility of thermally manipulating intracellular Ca2+ signalling. However, how the thermosensitive Ca2+ signalling would behave, particularly in multicellular systems, remains elusive. In this study, to extend the ability of the spatiotemporal temperature control by optical microheating technologies, we propose holographic heating microscopy. Water-absorbable infrared (IR) laser light is modulated by a reflective liquid crystal on a silicon spatial light modulator (LCOS-SLM). A computer-generated hologram displayed on the LCOS-SLM modulates the spatial phase pattern of the IR laser light to generate predesigned temperature gradients at the microscope focal plane. The holographic heating microscopy visualises how thermosensitive Ca2+ signalling is generated and propagated in MDCK cells, rat hippocampal neurons, and rat neonatal cardiomyocytes. Moreover, the optical control of the temporal temperature gradient reveals the cooling-rate dependency of Ca2+ signalling in HeLa cells. These findings demonstrate the extended ability of holographic heating microscopy in investigating cellular thermosensitivities and thermally manipulating cellular functions. |
| 巻・号 | 25(22) |
| ページ | 5863-5874 |
| 公開日 | 2025-11-4 |
| DOI | 10.1039/d5lc00675a |
| PMID | 41048005 |
| MeSH | Animals Calcium / metabolism Calcium Signaling* Dogs HeLa Cells Holography* / instrumentation Humans Madin Darby Canine Kidney Cells Microscopy* / instrumentation Microscopy* / methods Myocytes, Cardiac / cytology Myocytes, Cardiac / metabolism Neurons / cytology Neurons / metabolism Rats Temperature* |
| IF | 6.774 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NDCK(RCB0995) HeLa(RCB0007) |