Reference - Detail
| RRC ID | 86407 |
|---|---|
| Author | Isomura A, Asanuma D, Kageyama R. |
| Title | Synchronization of the segmentation clock using synthetic cell-cell signaling. |
| Journal | Genes Dev |
| Abstract |
During vertebrate development, the segmentation clock drives oscillatory gene expression in the presomitic mesoderm (PSM), leading to the periodic formation of somites. Oscillatory gene expression is synchronized at the cell population level; inhibition of Delta-Notch signaling results in the loss of synchrony and the fusion of somites. However, it remains unclear how cell-cell signaling couples oscillatory gene expression and controls synchronization. Here, we report that synthetic cell-cell signaling using designed ligand-receptor pairs can induce synchronized oscillations in PSM organoids. Optogenetic assays uncovered that the intracellular domains of synthetic ligands play key roles in dynamic cell-cell communication. Oscillatory coupling using synthetic cell-cell signaling recovered the synchronized oscillation in PSM cells deficient for Delta-Notch signaling; nonoscillatory coupling did not induce recovery. This study reveals the mechanism by which ligand-receptor molecules coordinate the synchronization of the segmentation clock and provides a way to program temporal gene expression in organoids and artificial tissues. |
| Volume | 40(1-2) |
| Pages | 124-141 |
| Published | 2026-1-5 |
| DOI | 10.1101/gad.352538.124 |
| PII | gad.352538.124 |
| PMID | 41102018 |
| PMC | PMC12758139 |
| MeSH | Animals Biological Clocks* / genetics Cell Communication* / genetics Gene Expression Regulation, Developmental* Ligands Mice Optogenetics Organoids / metabolism Receptors, Notch / genetics Receptors, Notch / metabolism Signal Transduction* / genetics Somites / cytology Somites / embryology Somites / metabolism |
| IF | 9.527 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | E14tg2a(AES0135) |