論文 - 詳細
| RRC ID | 86423 |
|---|---|
| 著者 | Kato I, Hagiwara Y, Asano M, Inoue J, Yamazaki Y, Yuguchi M, Yamada A, Tsunoda M, Imaoka S. |
| タイトル | Structural truncation of IL-1R2 enhances the anti-inflammatory activity of HeLa cells. |
| ジャーナル | Cell Struct Funct |
| Abstract |
Interleukin-1 receptor type 2 (IL-1R2) functions as a decoy receptor that suppresses IL-1-induced inflammatory signaling. Both membrane-bound IL-1R2 (WT IL-1R2) and its soluble form (sIL-1R2) bind interleukin-1α (IL-1α) at the cell surface or in the extracellular space, thereby inhibiting downstream signaling. However, the anti-inflammatory role of IL-1R2 varies depending on the cellular context and receptor structure. In this study, we generated two IL-1R2 deletion mutants-ΔTM, lacking the transmembrane domain, and ΔTMCP, lacking both the transmembrane and cytoplasmic domains-and compared their functions with those of WT IL-1R2 in HeLa cells. Western blotting, immunoprecipitation, and enzyme-linked immunosorbent assay were used to assess receptor expression, IL-1α binding, and IL-1β-induced interleukin-8 (IL-8) production, respectively. Both ΔTM and ΔTMCP were secreted more efficiently than WT IL-1R2. WT IL-1R2 exhibited weak intracellular interaction with IL-1α, whereas the deletion mutants showed minimal binding. WT IL-1R2 most effectively suppressed IL-1α extracellular release; however, ΔTM and ΔTMCP also reduced secretion. Notably, both deletion mutants suppressed IL-1β-induced IL-8 production more effectively than WT IL-1R2, indicating enhanced extracellular decoy activity. These findings demonstrate that structural modifications of IL-1R2 influence its function as a decoy receptor, and the enhanced inhibitory effects of the deletion mutants on IL-1 signaling provide new insight into the anti-inflammatory potential of soluble IL-1R2 in non-immune cells.Key words: Interleukin-1, Interleukin-1 receptor type 2, decoy receptor, Transmembrane, Soluble interleukin-1 receptor type 2. |
| 公開日 | 2025-12-12 |
| DOI | 10.1247/csf.25136 |
| PMID | 41391869 |
| MeSH | HeLa Cells Humans Inflammation Interleukin-1alpha / metabolism Interleukin-1beta / metabolism Interleukin-1beta / pharmacology Interleukin-8 / metabolism Receptors, Interleukin-1 Type II* / chemistry Receptors, Interleukin-1 Type II* / genetics Receptors, Interleukin-1 Type II* / metabolism Signal Transduction |
| IF | 2.429 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.8 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |
| 遺伝子材料 | Genome Network Project (GNP) Human cDNA IRAK082H06 (HGX032974) |