論文 - 詳細
| RRC ID | 86450 |
|---|---|
| 著者 | Kawai-Toyooka H, Ono M, Hamaji T, Nozaki H, Hirono M. |
| タイトル | Synergistic effects of proteinaceous pheromone and nitrogen starvation on male gametogenesis in the anisogamous volvocine alga Eudorina. |
| ジャーナル | PLoS One |
| Abstract |
In volvocine algae, gametogenesis is triggered by different cues depending on the species and their sexual systems. In isogamous unicellular organisms such as Chlamydomonas reinhardtii, which produce gametes of equal size and morphology, nitrogen depletion induces gametogenesis. In contrast, in oogamous multicellular species of the genus Volvox, which produce large, immobile eggs and small motile sperm, male gametogenesis is induced by a sex pheromone secreted by sperm packets (SPs), i.e., bundles of male gametes. Eudorina, an anisogamous multicellular volvocine alga that produces motile gametes of different sizes, is known to form SPs under nitrogen-depleted conditions. Intriguingly, a pheromone-like factor, present in male conditioned medium (CM), has also been implicated in SP formation. To clarify the relative contributions of nitrogen starvation and pheromone signaling, we conducted semi-quantitative analyses of SP induction using synchronously cultured male colonies of Eudorina. When CM was added to male cultures during an early maturation stage, most colonies formed SPs regardless of nitrogen availability. However, when the CM was diluted 100- to 100,000-fold, SP formation was significantly more efficient under nitrogen-depleted conditions than under nitrogen-replete conditions. Notably, SPs never formed without the addition of CM, even in a nitrogen-depleted medium. The SP-inducing activity of the CM was found to markedly decrease with protease treatment. These findings suggest that spermatogenesis in Eudorina is induced by a proteinaceous sex pheromone secreted by male colonies, and that nitrogen depletion, while not essential, enhances this pheromone activity. |
| 巻・号 | 20(11) |
| ページ | e0326066 |
| 公開日 | 2025-1-1 |
| DOI | 10.1371/journal.pone.0326066 |
| PII | PONE-D-25-28220 |
| PMID | 41270121 |
| PMC | PMC12637917 |
| MeSH | Chlorophyta* / metabolism Chlorophyta* / physiology Culture Media, Conditioned / pharmacology Gametogenesis* / drug effects Nitrogen* / deficiency Nitrogen* / metabolism Pheromones* / metabolism Pheromones* / pharmacology |
| IF | 2.74 |
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オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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| 藻類 | NIES-2734 NIES-2735 NIES-4100 NIES-4564 NIES-4565 NIES-4567 NIES-4568 |