論文 - 詳細
| RRC ID | 86537 |
|---|---|
| 著者 | Meile L, Alonso-Tolo G, Ferreiro-Eiras Z, Jiang X, Burén S, Rubio LM. |
| タイトル | A high-throughput heterologous expression platform for plant synthetic biology based on Arabidopsis suspension cells. |
| ジャーナル | J Exp Bot |
| Abstract |
Efficient heterologous expression platforms are essential for plant synthetic biology, particularly for engineering complex multigene pathways. Here, we establish a high-throughput system for transient and stable transformation of Arabidopsis thaliana suspension cells using plant cell pack infiltration. This method requires no specialized equipment or consumables and is compatible with several cell lines. It enables rapid generation of 100 g of transgenic cells within two weeks and allows expression of at least 6 stacked genes from a single construct. We characterized constitutive promoters for gene expression in Arabidopsis cells and validated plastid targeting peptides. A library of homologs of the nitrogenase iron-molybdenum cofactor maturase NifB was screened for expression and solubility and several archaeal variants suitable for plant expression were identified. We further engineered stable cell lines expressing up to six genes, encoding the NifB module components NifU, NifS, FdxN, and NifB, demonstrating that the newly developed platform integrates into an established workflow for nitrogenase engineering. The platform accelerates design-build-test cycles and facilitates the production of unstable and low-abundance proteins that require large amounts of transgenic biomass. It represents a versatile and scalable tool for advancing synthetic biology and for tackling major biotechnological challenges, such as biological nitrogen fixation. |
| 公開日 | 2025-12-23 |
| DOI | 10.1093/jxb/eraf545 |
| PII | 8402423 |
| PMID | 41432110 |
| IF | 5.908 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 2.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 rpc00050 |