論文 - 詳細
| RRC ID | 86699 |
|---|---|
| 著者 | He Y, Guo Y, Su G. |
| タイトル | Protection of GAP-43 gene-modified bone marrow mesenchymal stem cells on retinitis pigmentosa. |
| ジャーナル | J Transl Med |
| Abstract |
BACKGROUND:To observe the therapeutic effects of growth associated protein-43 (GAP 43) gene modified rat bone marrow mesenchymal stem cells (BMSCs) on experimental retinal degeneration. METHODS:A total of 80 male Royal College of Surgeons (RCS) rats were divided into three groups at postnatal 21 (P21) randomly. A cell suspension of 5 × 104 BMSCs modified with GAP-43 in 2 µl PBS was injected into the subretinal space of BMSCs + GAP-43 group rats, BMSCs group rats received5 × 104 BMSCs in 2 µl PBS and PBS group rats received 2 µl PBS. Photoreceptor necrosis and apoptosis was assessed by HE staining and Terminal-deoxynucleotidyl Transferase Mediated Nick End Labeling (TUNEL) detection. The expression of GAP-43, Glutamine synthetase (GS), rhodopsin (RHO), Caspase-8 and Caspase-9 was analyzed by immunofluorescence, western blot. RESULTS:Transplantation of GAP-43-modified BMSCs into the subretinal cavity of RCS rats revealed that: retinal GAP-43 expression was significantly elevated (P < 0.05); Rhodopsin (Rho) expression, a photoreceptor marker, was upregulated 1.5-fold (P < 0.05), and the thickness of the outer nuclear layer increased to 79.78 ± 6.83 μm (control 29.92 ± 4.17 μm, P < 0.01); The apoptosis rate decreased to 41.9% (control 63.8%, P < 0.01), and Caspase-8/9 activation was inhibited; Müller cell proliferation was reduced (GS decreased, P < 0.05). suggesting that GAP-43-modified BMSCs delayed retinal degeneration through anti-apoptosis and pro-photoreceptor survival. CONCLUSIONS:The results suggest that GAP-43 gene modified BMSCs has the therapeutic effect on the early stage of retinal degeneration, which might make more photoreceptors preserved. |
| 巻・号 | 24(1) |
| ページ | 153 |
| 公開日 | 2026-1-9 |
| DOI | 10.1186/s12967-026-07685-2 |
| PII | 10.1186/s12967-026-07685-2 |
| PMID | 41507979 |
| PMC | PMC12882432 |
| MeSH | Animals Apoptosis Bone Marrow Cells* / cytology Bone Marrow Cells* / metabolism GAP-43 Protein* / genetics GAP-43 Protein* / metabolism Male Mesenchymal Stem Cell Transplantation* Mesenchymal Stem Cells* / cytology Mesenchymal Stem Cells* / metabolism Rats Retinitis Pigmentosa* / pathology Retinitis Pigmentosa* / therapy Rhodopsin / metabolism |
| IF | 4.124 |
| リソース情報 | |
| ラット | RCS/Kyo (StrainID=27) |