論文 - 詳細
| RRC ID | 86753 |
|---|---|
| 著者 | Paatero I, Sauteur L, Lee M, Lagendijk AK, Heutschi D, Wiesner C, Guzmán C, Bieli D, Hogan BM, Affolter M, Belting HG. |
| タイトル | Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction. |
| ジャーナル | Nat Commun |
| Abstract |
Angiogenesis and vascular remodeling are driven by extensive endothelial cell movements. Here, we present in vivo evidence that endothelial cell movements are associated with oscillating lamellipodia-like structures, which emerge from cell junctions in the direction of cell movements. High-resolution time-lapse imaging of these junction-based lamellipodia (JBL) shows dynamic and distinct deployment of junctional proteins, such as F-actin, VE-cadherin and ZO1, during JBL oscillations. Upon initiation, F-actin and VE-cadherin are broadly distributed within JBL, whereas ZO1 remains at cell junctions. Subsequently, a new junction is formed at the front of the JBL, which then merges with the proximal junction. Rac1 inhibition interferes with JBL oscillations and disrupts cell elongation-similar to a truncation in ve-cadherin preventing VE-cad/F-actin interaction. Taken together, our observations suggest an oscillating ratchet-like mechanism, which is used by endothelial cells to move over each other and thus provides the physical means for cell rearrangements. |
| 巻・号 | 9(1) |
| ページ | 3545 |
| 公開日 | 2018-8-31 |
| DOI | 10.1038/s41467-018-05851-9 |
| PII | 10.1038/s41467-018-05851-9 |
| PMID | 30171187 |
| PMC | PMC6119192 |
| MeSH | Actins / metabolism* Animals Animals, Genetically Modified Antigens, CD / physiology* Cadherin 5 Cadherins / physiology* Cell Communication / physiology Cell Movement / physiology* Embryo, Nonmammalian Endothelial Cells / physiology* Intercellular Junctions / physiology Pseudopodia / physiology* Zebrafish Proteins / metabolism Zonula Occludens-1 Protein / metabolism |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | UAS:RFP |