| RRC ID |
86753
|
| 著者 |
Paatero I, Sauteur L, Lee M, Lagendijk AK, Heutschi D, Wiesner C, Guzmán C, Bieli D, Hogan BM, Affolter M, Belting HG.
|
| タイトル |
Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Angiogenesis and vascular remodeling are driven by extensive endothelial cell movements. Here, we present in vivo evidence that endothelial cell movements are associated with oscillating lamellipodia-like structures, which emerge from cell junctions in the direction of cell movements. High-resolution time-lapse imaging of these junction-based lamellipodia (JBL) shows dynamic and distinct deployment of junctional proteins, such as F-actin, VE-cadherin and ZO1, during JBL oscillations. Upon initiation, F-actin and VE-cadherin are broadly distributed within JBL, whereas ZO1 remains at cell junctions. Subsequently, a new junction is formed at the front of the JBL, which then merges with the proximal junction. Rac1 inhibition interferes with JBL oscillations and disrupts cell elongation-similar to a truncation in ve-cadherin preventing VE-cad/F-actin interaction. Taken together, our observations suggest an oscillating ratchet-like mechanism, which is used by endothelial cells to move over each other and thus provides the physical means for cell rearrangements.
|
| 巻・号 |
9(1)
|
| ページ |
3545
|
| 公開日 |
2018-8-31
|
| DOI |
10.1038/s41467-018-05851-9
|
| PII |
10.1038/s41467-018-05851-9
|
| PMID |
30171187
|
| PMC |
PMC6119192
|
| MeSH |
Actins / metabolism*
Animals
Animals, Genetically Modified
Antigens, CD / physiology*
Cadherin 5
Cadherins / physiology*
Cell Communication / physiology
Cell Movement / physiology*
Embryo, Nonmammalian
Endothelial Cells / physiology*
Intercellular Junctions / physiology
Pseudopodia / physiology*
Zebrafish Proteins / metabolism
Zonula Occludens-1 Protein / metabolism
|
| IF |
12.121
|
| リソース情報 |
| ゼブラフィッシュ |
UAS:RFP |
